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Heavy Metal Index

Comparative Study on the Distribution of Essential, Non-Essential Toxic, and Other Elements across Trophic Levels in Various Edible Aquatic Organisms in Sri Lanka and Dietary Human Risk Assessment

Source

Wickrama-Arachchige and colleagues measured 36 elements in edible aquatic organisms sold in Sri Lankan local fish markets.

Page snapshot
Cited by14 pages
Metals measured15
Evidence tierB
Year2022

Overview

Wickrama-Arachchige and colleagues measured 36 elements in edible aquatic organisms sold in Sri Lankan local fish markets. The main PDF reports trophic-level summaries, tuna muscle-type comparisons, a yellowfin-tuna mercury comparison table, and yellowfin-tuna risk calculations. Species-level concentration tables are named as supplementary Tables S2 and S3; they were not bundled in the local PDF, so this page preserves the exact main-PDF values and flags the supplement as required for future species-level extraction.

The study reports total mercury measured by a mercury analyzer. For the human-health calculation, the authors assume all total Hg in yellowfin tuna is methylmercury; this page records that assumption as a source assumption and does not relabel the measured occurrence table as directly speciated MeHg.

Key numbers

Sample frame and methods:

FieldValue
Individual organisms80
Fresh samples36
Species29
Offshore pelagic species18
Coastal/estuarine species11
Yellowfin tuna samples7
Skipjack tuna samples3
Element concentration basismg/kg wet weight
EDXRF detection limit used for elements other than Hg1.0 mg/kg dry weight; half detection limit (0.5 mg/kg) used for values below detection in analysis
Yellowfin tuna consumption assumption3.8 g/day (0.0038 kg/day)
Adult body weight assumption60 kg

Element groups used by the paper:

GroupElements
Essential but toxic in excess amount (EBTEs)Sn, Fe, Cu, Cr, Zn, Se
Non-essential toxic elements (NETs)As, Sb, Cd, Hg, Pb
Other elements detected/discussedNi, Mg, Al, Si, P, S, Cl, K, Ca, Ti, Mn, Co, Br, Rb, Sr, Te, I, Hf, Ta, Pt, Au

Main-PDF trophic-level summaries:

EcosystemElement groupLower trophic levelMiddle trophic levelHigher trophic levelSource pattern
Offshore pelagicEBTEs31.6 mg/kg31.0 mg/kg11.4 mg/kgHTL < MTL < LTL
Offshore pelagicNETs1.49 mg/kg1.86 mg/kg1.89 mg/kgLTL < MTL < HTL
Offshore pelagicOther elements7610.2 mg/kg10012.3 mg/kg6503.8 mg/kgHTL < LTL < MTL
Coastal/estuarineEBTEs23.6 mg/kg13.5 mg/kg8.82 mg/kgHTL < MTL < LTL
Coastal/estuarineNETs0.64 mg/kg1.99 mg/kg0.84 mg/kgLTL < HTL < MTL
Coastal/estuarineOther elements8457.1 mg/kg8052.8 mg/kg7296.3 mg/kgHTL < MTL < LTL

Element-order summaries reported in the main text:

EcosystemGroupIncreasing concentration order
Offshore pelagicEBTEsCr < Se < Cu < Sn < Zn < Fe
Offshore pelagicNETsPb < Sb < Cd < Hg < As
Offshore pelagicOther abundant elementsSr < Br < Si < Al < Mg < Ca < P < Cl < S < K
Coastal/estuarineEBTEsCr < Se < Cu < Sn < Fe < Zn
Coastal/estuarineNETsCd < Hg < Pb < Sb < As
Coastal/estuarineOther abundant elementsBr < Sr < Si < Al < Mg < Ca < Cl < P < S < K

Maximum-allowable-limit exceedance signals reported in the main text:

EcosystemElementSource MALSpecies reported above MAL
Offshore pelagicCr0.1 mg/kg wet weightRastrelliger kanagurta, Mobula kuhlii, Auxis thazard
Offshore pelagicAs3 mg/kgMobula kuhlii, Katsuwonus pelamis (1.5 kg), Rhizoprionodon acutus
Offshore pelagicCd0.05 mg/kgLoligo duvauceli
Offshore pelagicPb0.2 mg/kgSardinella albella
Coastal/estuarineAs3 mg/kgLethrinus nebulosus, Lutjanus rivulatus

Tuna muscle-type summaries from Figure 4 and the text:

SpeciesMuscle typeEBTEsNETsOther elementsHg note
Yellowfin tuna (Thunnus albacares)White muscle11.5 +/- 3.65 mg/kg1.4 +/- 0.45 mg/kg10549.3 +/- 2978.4 mg/kgThe paper reports nearly equal Hg between white and red muscles
Yellowfin tuna (Thunnus albacares)Red muscle37.1 +/- 16.6 mg/kg1.93 +/- 0.65 mg/kg11809.7 +/- 2122.6 mg/kgThe paper reports nearly equal Hg between white and red muscles
Skipjack tuna (Katsuwonus pelamis)White muscle29.8 +/- 8.75 mg/kg2.45 +/- 0.45 mg/kg11911.9 +/- 3836 mg/kgThe paper reports higher Hg in white than red muscle
Skipjack tuna (Katsuwonus pelamis)Red muscle76.6 +/- 6.77 mg/kg3.1 +/- 2.18 mg/kg10426.3 +/- 556.0 mg/kgThe paper reports higher Hg in white than red muscle

Yellowfin tuna total-mercury findings:

FindingValue
Yellowfin tuna Hg sample count7
Mean Hg in yellowfin tuna0.16 +/- 0.16 mg/kg wet weight
Hg range in yellowfin tuna0.02-0.43 mg/kg wet weight
Mean length/weight in the study’s Table 1 comparison row118 cm / 27 kg
Hg-body-weight regressionR2 = 85.7
Hg-body-weight regression p value0.003
ANOVA MS for Hg-body-weight relationship0.126
ANOVA F for Hg-body-weight relationship29.97
ANOVA DF for Hg-body-weight relationship1

Table 1 compares the study’s yellowfin-tuna Hg results with literature values:

Mean HgRangeMean length/weightMethodCountryOceanReference
0.16 +/- 0.16 mg/kg0.02-0.43 mg/kg118 cm / 27 kgMercury analyzer (MA 3000, USA)Sri LankaIndian OceanThis study
0.30 +/- 0.18 mg/kg0.021-0.98 mg/kg123.4 cm / 45.3 kgCold vapor system atomic absorption spectrophotometrySri LankaIndian Ocean[24]
0.26 +/- 0.29 mg/kg--Atomic absorption spectrophotometrySri LankaIndian Ocean[19]
0.51 +/- 0.33 mg/kg-74.3 +/- 11.4 cmAtomic absorption spectrophotometryBaja California Sur, CaliforniaEastern Pacific Ocean[69]
0.98 +/- 0.69 mg/kg-92.2 +/- 19.5 cmAtomic absorption spectrophotometryEquatorial ZoneEastern Pacific Ocean[70]
0.51 +/- 0.32 mg/kg-22 kg / 109 cmAdvanced mercury analyzer (combustion analyzer ALTEC 254)Mozambique channelWestern Indian Ocean[71]
0.70 +/- 0.49 mg/kg-24 kg / 104 cmAdvanced mercury analyzer (combustion analyzer ALTEC 254)Reunion IslandWestern Indian Ocean[71]
0.77 mg/kg0.45-1.52 mg/kg wet weight29.0-50.8 kgInductively coupled plasma mass spectrometrySouth AfricaSouth Atlantic[29]
2.75 +/- 0.98 mg/kg dry weight1.51-4.49 ng/g dry weight138.8 cm forked length / 58.7 kgCold vapor atomic absorption spectroscopyWest AfricaNorth Atlantic Ocean[28]
159 +/- 79 ng/g wet weight48-500 ng/g wet weight92 +/- 28 cm forked length / 16.8 +/- 13.3 kgCold vapor atomic absorption spectrometryBrazilEquatorial Atlantic Ocean[67]

Yellowfin-tuna risk calculations reported in the main PDF:

MetricValue
THQ-As0.22 +/- 0.07
THQ-Sb0.019 +/- 0.003
THQ-Cd0.0067 +/- 0.001
THQ-Hg0.024 +/- 0.001
THQ-Pb0.0016 +/- 0.0003
Hazard index0.27
EDI of NETs for Sri Lankan adults9.38 x 10^-5 +/- 1.58 x 10^-5 mg/kg bw/day
PTWI-As0.47 +/- 0.13 ug/kg bw/w
PTWI-Sb, PTWI-Cd, and PTWI-Pb0.05 +/- 0.008 ug/kg bw/w
PTWI-M-Hg0.05 +/- 0.02 ug/kg bw/w

Methods (brief)

The authors purchased commonly consumed edible aquatic organisms from Sri Lankan local fish markets. Samples were transported on ice, dissected, and stored below -40 C. Heads, fins, and viscera were removed from small to medium specimens; approximately 200 g of muscle from the anterior dorsal region near the pectorals was collected from large fish. Tuna red and white muscles were separated for yellowfin and skipjack comparisons.

Most elements were analyzed using a Rigaku EDXL-300 energy-dispersive X-ray fluorescence spectrometer after freeze-drying, homogenization, binder mixing, and pellet preparation. Mercury was analyzed separately using a NIC MA-3000 Mercury Analyzer, with two replicates from one sample.

Stable isotope analysis was used to assign trophic-level patterns. Human-risk calculations for yellowfin tuna used 3.8 g/day yellowfin-tuna consumption for Sri Lankan adults and 60 kg body weight. The authors assumed all total Hg in yellowfin tuna was present as methylmercury for risk-assessment comparison.

Implications

This source supports broad seafood occurrence context for Sri Lankan market fish and shellfish, with a direct yellowfin-tuna total-Hg value and main-text summary signals for arsenic, cadmium, lead, chromium, and other elements across edible aquatic organisms. The main-PDF values are especially useful for fish-route context and for documenting that yellowfin-tuna Hg rose with body weight in this sample set.

The paper is not infant-formula evidence despite being located in an infant-formula methylmercury pull folder. It does not measure methylmercury directly; the MeHg risk calculation is derived from a source assumption that total Hg in tuna represents methylmercury. Species-level extraction should use the supplementary Tables S2-S3 if they are retrieved later.

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Verification notes

  • Identity check: DOI 10.3390/toxics10100585, raw handle RPMEHG_10-3390-toxics10100585, candidate cite-key wickramaarachchige2022-sri-lanka-aquatic-elements, and SHA-256 7b70522aa3955dce324aada87be0f2fce09b81e276afdc181b9f4fd45a91dc72 were searched before creation; no existing clean post-2026-05-14 source page was found.
  • Full-PDF read performed from the 19-page MDPI PDF. Main-PDF Table 1, Figures 1-5, and all main-text numeric summaries were checked once from pdftotext -layout and a second time against the extracted layout text before commit. The source’s supplementary Tables S1-S4 are named by the PDF but were not bundled in the local PDF.
  • Brand firewall: the source reports species- and muscle-level market seafood values, not branded commercial products.
  • HMTc firewall: page records source facts only; no certification threshold, percentile, or pass/fail calculation is derived from this source.

Update history

The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.

CommitDateChangeDescription
3171d062026-08-02major1 section added
bc84bfc2026-08-02major6 sections added; narrative text revised