Chen and colleagues fed C57BL/6 mice diets with 0, 30, 150, or 600 mg/kg zinc for 4 weeks and for 8 weeks, then sequenced cecal 16S rRNA and measured metabolites by gas chromatography mass spectrometry. The WikiBiome corpus note states that both low zinc and high zinc changed the gut microbiota, with different patterns.
Key numbers
The note states that a 4-week diet with 0 mg/kg zinc increased Proteobacteria and Desulfovibrio. An 8-week diet with 150 mg/kg zinc suppressed total short-chain fatty acids, butyric acid, acetic acid, and short-chain-fatty-acid-producing genera. A 600 mg/kg diet decreased Shannon diversity. Melainabacteria had an area under the curve above 0.85 as a phylum-level marker and was negatively correlated with serum zinc. Desulfovibrio sp. ABHU2SB had a receiver-operating-characteristic area under the curve of 0.8 to 0.91. The note does not give the number of mice.
Evidence fitness
The record supports a mouse diet finding about zinc and cecal microbiota. It does not support a concentration of zinc in any food other than the experimental diets, and it does not state a limit.
Limitations
This page records the WikiBiome corpus note. It is not a new extraction from a local copy of the paper. The mouse count is not in that note.
Related evidence
Update history
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