Overview
Mondal and colleagues developed a label-free colorimetric biosensor using gold nanoparticles (13 nm, citrate-capped) conjugated to an anti-SEB DNA aptamer for the detection of staphylococcal enterotoxin B (SEB) in food. SEB binding to the aptamer displaces the citrate salt and induces AuNP aggregation with a red-to-blue color shift. Gold nanoparticles are the analytical signal element; SEB is the target analyte. Validated matrices include commercial whole milk, apple juice, and pomegranate juice.
Key numbers
Visual LOD: 50 ng/mL SEB. Spectrometric LOD: 0.5 ng/mL. Detection time: a few minutes. Recoveries: 88 to 96% across food matrices. Spectrometric result at 520 nm (dispersed AuNPs) versus 620 nm (aggregated AuNPs).
Implications for the wiki
This paper contributes to Gold as documentation of AuNP-aptamer colorimetric platforms applied to foodborne toxin detection in multiple food matrices including dairy and juices. No food occurrence data for gold or any heavy metal are provided.
Related evidence
Update history
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