Shrimp (Penaeus semisulcatus, Decapoda:Penaeidae) from the

Overview

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Key numbers

The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:

enderun Bay, where male shrimps exhibited concentrations (1.209 mg/kg wet weight)
across all stations, with several samples surpassing EU maximum levels (0.50 mg/kg).
was precisely weighed and transferred into digestion vessels. A mixture of 5 mL of 65%
HNO3 and 1 mL of 37% HCl was added to each vessel (the addition of HCl ensured the
and reference doses (RfD) used in these calculations are summarized in Table 1.
version factor of 10% was applied to the total arsenic concentrations for the CR calculations,
and Pb were 1.5, 0.01, 0.5, 1.7, and 0.38 mg kg−1 day−1 , respectively. The acceptable
Table 1. Parameters and values used for THQ and CR calculations.
Cu = 0.04; Fe = 0.7; Mn = 0.14; Zn = 0.3; Hg = 0.0001; Se = 0.005;
centration (mg/kg) of each element by its respective atomic weight (Se: 78.96 g/mol; Hg:
Table 2. Mean weights (g), total lengths (cm), and meat yield (%) of green tiger shrimp (P. semisulcatus).
multiple range test (p < 0.05); values sharing the same superscript letter are not significantly different (p > 0.05).
58.00 ± 12.66 g, significantly surpassing the males (33.50 ± 5.35 g). As shown in Table 2,
tably, males at Station 1 and Station 4 exhibited the highest meat yield percentages (59.84%
TL, MW, and % MY columns in Table 2, reflecting the absence of significant station-based
The mean concentrations of 12 analyzed metals (As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb,
Table 3. Mean concentrations (mg/kg wet weight ± SD) of analyzed metal(loid)s in the muscle tissue
1: Bozyazi; 2: Silifke; 3: Karatas; 4: Iskenderun. ♀: Female; ♂: Male; Values are expressed as Mean ± Standard
0.855. The mean As concentrations in the muscle tissue of P. semisulcatus ranged from
1.83 ± 0.22 mg/kg (Karatas, Female) to 5.66 ± 0.67 mg/kg (Silifke, Female). The highest
with males in Iskenderun reaching 5.61 ± 0.73 mg/kg (Figure 2). Specifically, the lowest
Figure 2. Mean As concentrations (mg/kg wet weight) in the muscle tissue of P. semisulcatus by
Cd level was measured in males (0.041 ± 0.005 mg/kg). Across all sampling stations and
sexes, Cd concentrations ranged from 0.014 ± 0.001 mg/kg to 0.041 ± 0.005 mg/kg.
(1.209 ± 0.15 mg/kg), which was substantially higher than the levels observed in females
at the same station (0.128 ± 0.01 mg/kg) (Figure 3). This significant difference indicates
(0.147 ± 0.02 mg/kg) and Iskenderun females (0.128 ± 0.01 mg/kg).
Figure 3. Mean Cd and chromium Cr concentrations (mg/kg wet weight) in the muscle tissue of
the model explains approximately 90.7% of the total variance in Cu concentrations.
(Karatas, Male) to 8.32 ± 1.00 mg/kg (Bozyazi, Male) (Figure 4). Notably, Cu concentrations
were distinctively higher in Bozyazi (7.97–8.32 mg/kg) compared to other stations, which
(18.18 ± 2.32 mg/kg), followed by Bozyazi males (18.05 ± 1.89 mg/kg). In contrast, the
lowest Fe levels were measured in Karatas females (0.32 ± 0.03 mg/kg) (Figure 5).
R2 = 0.998). Hg concentrations in Iskenderun (0.009 mg/kg) were approximately 9 times
higher than those measured in Bozyazi (0.001 mg/kg).
Figure 5. Mean Fe and Hg concentrations (mg/kg wet weight) in the muscle tissue of P. semisulcatus
significant (p = 0.141), which is reflected by the lack of differentiating superscripts in Table 3.
Figure 6. Mean Pb and Zn concentrations (mg/kg; wet weight) in the muscle tissue of P. semisulcatus
in Bozyazi females (16.44 ±1.99 mg/kg) and Iskenderun females (15.95 ± 2.05 mg/kg)
females (0.141 ± 0.01 mg/kg). Ni concentrations followed a similar pattern of geo-
(0.403 ± 0.05 mg/kg) and Bozyazi females (0.384 ± 0.04 mg/kg) (Figure 7).
Figure 7. Mean Mn, Ni, and Se concentrations (mg/kg wet weight) in the muscle tissue of

Methods (brief)

across all stations, with several samples surpassing EU maximum levels (0.50 mg/kg).
and metalloids in the edible tissues of female and male P. semisulcatus individuals collected
were collected using commercial trawling nets. To ensure statistical robustness and compare
biological variables, approximately 30 individuals were collected from each station, with
Immediately after collection, the samples were rinsed with ambient seawater to remove
mogenized. This procedure resulted in a total of 8 composite samples (4 stations × 2 sexes).
2.2. Microwave Digestion Procedure
Sample preparation was performed by acid decomposition of the tissue matrix using
a microwave digestion system (Berghof MSW-4, Eningen, Germany). From each of the
8 composite homogenized samples, approximately 250 mg (wet weight) of the tissue
was precisely weighed and transferred into digestion vessels. A mixture of 5 mL of 65%
The digestion process was carried out according to a specific multi-step thermal program:
Following digestion, the resulting solutions were diluted to a final volume of 10 mL
Spectrometry (ICP-MS) instrument (Perkin Elmer NexION 350X, Waltham, MA, USA). The
1.2 L/min, and nebulizer gas flow of 0.68 L/min. The sample uptake rate was maintained
acceptable limits. All samples were analyzed in triplicate to verify reproducibility. The
limits of detection (LOD) and quantification (LOQ) were calculated as three and ten times
of the analyzed samples, the Target Hazard Quotient (THQ) was calculated for individual

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Update history

The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.

Commit	Date	Change	Description
b01ec52c	2026-08-04	major	2 sections added
d49e450f	2026-08-03	major	5 sections added; narrative text revised