Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- posure to mining waste is associated with detoxification axes 9.50% and 3.30% of the total variance, respectively (R2X=
- for each species. In this process, feature-based molecular Supporting Information (Table S1 and Figures S27−S51).
- 56% were classified as peptides, 20% as phenolic compounds, and Cr, which reached contamination levels after the
- 12% as peptide-phenolic conjugates, and 12% remained environmental disaster.64 Specifically, an increase in Cd
- important discriminant metabolites, 32% corresponded to derivatives in enhancing plant resilience against stresses caused
- phenolic compounds, 16% to terpenoids, and 12% to by metalloids. These compounds are crucial in the synthesis of
- terpenoid-phenolic conjugates, while 40% could not be sulfur-rich peptides and amino acids, as well as nitrogenous
- Supporting Information (Table S2 and Figures S52−S76). provides complementary protection in which polyhydroxylated
- derivatives containing an acetylated lysine residue, and P. acetonitrile (solvent B), both containing 0.1% of formic acid.
- methylated flavonoid C-glycosides and prenylated phenolics. following gradient was employed: 0−1 min (10% B), 1−45
- deposited and registered in the José Baldini Herbarium at the intensity, 1.0E;6 m/z tolerance, 0.3 m/z or 0 ppm);
- searched at https://specieslink.net. All samples were individ- duration range, 0.2−2.0; RT wavelet range, 0.02−0.20), m/z
- ually dried over silica gel at ambient conditions, powdered center calculation − median, m/z range for MS2 scan pairing,
- and stored in sealed vials until further chemical extraction. tolerance, 0.5 m/z or 0 ppm; retention time tolerance, 0.2 min
- data were exported as a *.mgf file and *.csv quantification table 129 Da for aliphatic glutathione residues, 178 Da for
- *.csv quantification table, which was previously log10-trans- and conjugated systems, which typically exhibit two maximum
- not search; maximum analog search mass difference, 100 Da; molecular networks, and tables with spectrometric and
- (8) Orlando, M. T. D.; Galvão, E. S.; Cavichini, A. S.; Rangel, C. V. Asteraceae). Phytologia 1992, 73, 65−76.
Methods (brief)
- Using an untargeted liquid chromatography-tandem mass spectrometry (LC-MSn) metabolomics workflow integrated with
- score plot, red dots represent the samples of individuals growing in areas affected by the Fundão dam collapse, and green dots represent samples of
- discriminating samples between affected and unaffected areas for each plant species. R2Y, goodness of fit for the Y variables; Q2, goodness of
- Figure 3. Metabolite classes of the top 25 selected discriminants for (a) Vernonanthura polyanthes and (b) Piper aduncum individuals collected in
- and P. aduncum collected in tailings-affected and unaffected polyanthes, PC1 and PC2 explained 70.40% and 5.73% of the
- aduncum individuals collected from areas affected by the the top discriminant metabolites.
- separation between affected and unaffected samples, we peptides with an acetylated lysine moiety were also annotated
- an intuitive way of identifying potential metabolic pathways polyanthes individuals collected in areas affected by the Fundão
- affected areas and one in plants from unaffected areas). in V. polyanthes individuals collected in areas affected by the
- act as discriminants for P. aduncum individuals collected in glutathione derivatives were characterized by the presence of
- discriminant metabolites only in individuals collected from lysine acetylation is an important post-translational modifica-
- metabolites exclusively in individuals collected from areas a flavonoid glycoside without an attached OCH3 group
- metabolites for P. aduncum individuals collected in areas scavenging, while O-methylation/prenylation optimizes local-
- revealed species-specific metabolic strategies in response to Billerica, MA, USA). The samples were analyzed using a
- Plant Collection, Sample Preparation, and LC-MSn
- Leaves of P. aduncum and V. polyanthes were collected in areas The molecular networking and multivariate statistical analyses
- conducted in August 2018. For each plant species, we collected following parameters: mass detection, mass detector −
- 30 individuals per species were sampled). Plant materials were scans, 5; group intensity threshold, 1.0E;5 min highest
Implications
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Verification notes
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Update history
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