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Heavy Metal Index

Antibiotic Pollution Elevates Microbial Methylmercury Production

Source

This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus.

Page snapshot
Cited by6 pages
Metals measured2
Evidence tierB
Year2026

Overview

This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.

Key numbers

The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:

  • bacteria frequently exhibit coselected resistance.24 While the Pseudodesulfovibrio mercurii ND132 (Table S1) was used in this study
  • transporter gene transcription and forming specific Hg(II) environment21,30). Enriched stable isotope techniques were employed
  • appropriate antibiotics, the risk of elevated MeHg pollution in obtain a final concentration of 2.5 nM, within the concentration range
  • TOB (i). Error bands or bars represent ±1 standard deviation of replicates (n = 3). Italic lowercase letters indicate a significant difference (p <
  • concentrated HCl was added to give a final concentration of 0.4% (v/ chelate structural ions in the peptidoglycan layer. After a 1 min
  • Information (Table S4). mercury fluorescent probe (Xi’an Qiyue Biology, China). Sub-
  • Technologies, USA). Clustering of the index-coded samples was showed no detectable loss during the 12 h incubation period
  • exposure concentrations of 50 and 500 μg L−1 (Figure S1). environment becomes less inhabitable for methylating micro-
  • deviation of replicates (n = 3). The black line and gray band in (a) represent the mean value ±1 standard deviation of intracellular Hg(II) in cells
  • presence of 2.5 nM dissolved Hg(II). Error bars represent ±1 standard deviation of replicates (n = 3). Italic lowercase letters indicate a significant
  • qPCR) (Table S2), increased in the presence of ciprofloxacin complexes formed between Hg(II) and natural organic matter,
  • ciprofloxacin and Hg(II)−tetracycline (Figure S8 and Table

Methods (brief)

  • predetermined time interval, triplicate samples were collected and 500 mM ethylenediaminetetraacetic acid (EDTA) was added to
  • v), to terminate the reactions and preserve the samples for subsequent reaction with EDTA, 4 mL of 700 mM sucrose was added to the cell
  • EPA Method 163034 using coupled cold vapor atomic fluorescence centrifugation at 20,000g for 10 min and washed twice to remove
  • coupled plasma mass spectrometry (ICP-MS; NexION 2000, were then resuspended in 5 mL of a wash media. The bacterial
  • Whole cell culture samples (OD600 = 0.7 ∼ 0.9) were collected by fold at the corresponding concentrations (Figures S10 and
  • (Tianjin, China). Total RNA was extracted from the samples using
  • Technologies, USA). Clustering of the index-coded samples was showed no detectable loss during the 12 h incubation period
  • dissolved Hg(II) (Figure S3) in the presence of antibiotics (a). Single-cell−inductively coupled plasma−mass spectrometry (SC-ICP-MS)
  • plasma−mass spectrometry (SC-ICP-MS) showed that anti- probe is capable of penetrating into the spheroplasts of P.
  • order of Hg(II)−ciprofloxacin > Hg(II)−tetracycline > concentrations of Hg(II) in whole culture samples,
  • D: Mercury in Water by Oxidation, Purge and Trap, and Cold Vapor J. 2021, 15 (6), 1810−1825.
  • and Cold Vapor Atomic Fluorescence Spectrometry; U.S. Environmental (51) Zhang, T.; Kim, B.; Levard, C.; Reinsch, B. C.; Lowry, G. V.;
  • of Tetrabromobisphenol A on Anaerobic Digestion: Responses of

Implications

This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.

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Verification notes

  • Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing wiki/sources/ pages before creation.
  • Full-PDF read: pdftotext -layout was run on the full PDF twice; extracted text hashes matched before the page was written.
  • Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
  • Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
  • HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.

Update history

The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.

CommitDateChangeDescription
b01ec52c2026-08-04major2 sections added
d49e450f2026-08-03major5 sections added; narrative text revised