Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- C (PKC) (13–15). Proteins, being the molecular effectors of biological processes, are suitable
- Mercury chloride (HgCl2 ; >99.5% pure; CAS number 7487-94-7; Sigma-Aldrich, St.
- (for one hour and four days’ exposure, respectively) are 1.8 and 0.94 µg/L (ppb) (40). The
- only for Hg (EC number 231-106-7), for which it is set at 0.067 µg/L (ppb) (41). A stock
- solution of 1 mg/L Hg2+ was prepared by dissolving HgCl2 in filtered (0.22 µm) NSW
- and subsequently diluting the solution to the nominal concentration (10 µg/L). The Hg
- Tris-HCl, pH 7.8, 0.25 M sucrose, 1% (w/v) SDS (sodium dodecyl sulfate), 1 µg/mL pep-
- 5 mM NEM (N-ethylmaleimide), 40 µg/mL PMSF (phenylmethylsulfonyl fluoride), 0.1%
- resolved either by 10% (for HSP) or 12% (for phospho-p38 MAPK) SDS-polyacrylamide gel
- stripping buffer (62.5 mM Tris–HCl, pH 6.7, containing 10 mM β-mercaptoethanol and 2%
- defined as the activity causing 50% inhibition of cytochrome c reduction at 25 ◦ C.
- significant difference (HSD) tests. The confidence level was set at 99% (α = 0.01).
- protein (Figure 1 and Table S1). The GST exhibited significantly higher (p < 0.0001) values in
- protein; Figure 1 and Table S1). The GSR levels were significantly higher (p < 0.001)
- (4.16 ± 0.31 nmol/mg protein; Figure 1 and Table S1). The GPx exhibited significantly
- samples (11.34 ± 1.04 nmol/mg protein; Figure 1 and Table S1). The Se-GPx values are
- 10 µg/L of Hg andforaminiferal Amphistegina
- 0.01) reported as mean ±
- standard deviation (n = 5). Letters denote significant differences (p < 0.01) between groups.
- lessonii in samples exposed for 24 h at 10 µg/L of Hg and control samples (no added Hg). Data are
- lessonii in samples exposed for 24 h at 10 µg/L of Hg and control samples (no added Hg). Data
- reported as mean ± standard deviation (n = 5). Letters denote significant differences (p < 0.01) be-
- are reported as mean ± standard deviation (n = 5). Letters denote significant differences (p < 0.01)
- variation, heavy metals, salinity, etc. (93). HSPs mediate a wide range of cellular functions
- //www.mdpi.com/article/10.3390/biology11070960/s1: Table S1: Raw data of total superoxide
- bearing foraminiferal species Amphistegina lessonii; Table S2: Raw data of HSP-70 and p-p38 in the
-
- Weinmann, A.E.; Rödder, D.; Lötters, S.; Langer, M.R. Traveling through time: The past, present and future biogeographic range
-
- Weinmann, A.E.; Rödder, D.; Lötters, S.; Langer, M.R. Heading for new shores: Projecting marine distribution ranges of selected
-
- US EPA. National Recommended Water Quality Criteria—Aquatic Life Criteria Table (WWW Document). 2015. Available online:
- https://www.epa.gov/wqc/national-recommended-water-quality-criteria-aquatic-life-criteria-table (accessed on 2 March 2022).
Methods (brief)
- Individuals of A. lessonii were collected from rock pebbles at approximately 1–2 m
- (NSW), with a salinity of 40.4 and a pH of 8.15, collected from the sampling site. They were
- buffer (25). Specifically, samples were lysed with 1 mL of ice-cold lysis buffer (50 mM
- Nonidet-P40) and sonicated. Samples were boiled for 10 min and then centrifuged for
- sample buffer (0.5 M Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 4% 2-mercaptoethanol, 0.05%
- bromophenol blue), and samples (normalized to 30 µg protein content before loading) were
- (Quantity One software) that also calibrated the molecular weights of the sample bands
- performed utilizing known concentrations of GSSG instead of samples.
- Differences between control and treatment samples in the mean values of enzymes
- The SOD activity showed significantly higher (p < 0.0001) values in treatment samples
- (26.00 ± 3.50 U SOD/mg protein) than in control samples (8.65 ± 2.08 U SOD/mg protein;
- Figure 1 and Table S1). The GSH levels in the treatment samples (1.64 ± 0.15 GSH/mg protein)
- treated samples (23.70 ± 1.17 nmol/mg protein) than in control samples (3.25 ± 0.24 nmol/mg
- in treated samples (10.98 ± 0.27 nmol/mg protein) than those in control samples
- higher (p < 0.0001) levels in treated samples (22.92 ± 1.81 nmol/mg protein) than in control
- samples (11.34 ± 1.04 nmol/mg protein; Figure 1 and Table S1). The Se-GPx values are
- significantly higher (p < 0.0001) in treated samples (71.07 ± 5.62 nmol/mg protein) than
- peroxidase (Se-GPx) in the symbiont-bearing foraminiferal species Amphistegina lessonii in samples
Implications
This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.
Wiki pages this source may touch
- Shellfish (shrimp, crab, lobster, clams, oysters, mussels)
- Seaweed/kelp foods (nori, wakame, kombu, dulse — as food products)
- Mercury
- Mercury
- Cadmium
- Nickel
Verification notes
- Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing
wiki/sources/pages before creation. - Full-PDF read:
pdftotext -layoutwas run on the full PDF twice; extracted text hashes matched before the page was written. - Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
- Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
- HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.
Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.