Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- in the human organism following a therapeutic dose of DMSA (30 mg/kg). To avoid stability issues,
- consisting of a 1:1 mixture of Dulbecco’s Modified Eagle’s Medium and F-12, supplemented with 10%
- fetal bovine serum and 1% Pen-Strep mixture in a humidified incubator at 37 ◦ C and 5% CO2 .
- Appropriate controls for vehicle (ethanol 0.4%) and spontaneous MTT reduction by chelating agents
- 70%–80% confluence. Fresh medium was then supplied to cells followed by the addition of MeHg
- sodium pyrophosphate; 20 mM sodium β-glycerophosphate; 0.5% TritonX-100 and protease inhibitor
- SDS-PAGE on a 4%–12% Bis-Tris gel with MES running buffer (ThermoFisher® ), followed by transfer
- to a nitrocellulose membrane which was subsequently blocked with a 5% skimmed milk solution and
- Results in figures are presented as mean ± standard error (SE) (see Table S1 for details) of at least
- very significant at p Table S1 for details) of at least
- decrease in DTNB reduction (to 25% of control) (Figure 2a). None of the chelating agents caused aIndeed,
- MeHg (200 nM) resulted in a drop in activity to 48% of control of the level.
- MeHg (200 nM) resulted in a drop in activity to 48% of control level. Similar
- test concentrations, chelating agents could recover Trx system activity for levels between 70% and 90%
- the test concentrations, chelating agents could recover Trx system activity for levels between 70% and
- 90% of control activity with efficacy for BAL > DMSA > (15)aneN4S (Figure 2b).
- TrxR activity, whereas DMSA caused a 20% drop in activity
- TrxR activity, whereas DMSA caused a 20% drop in activity (p < (p < 0.01). Most importantly,
- Thioredoxin activity was less affected by exposure of SH-SY5Y to MeHg with a drop to 47% of
- for 24 h. All data points represent the mean ± SE of at least three independent experiments. Different
- 24 h. All data points represent the mean ± SE of at least three independent experiments. Different
- reduction assay was less exuberant (50%) than observed with the DTNB assay (10). The insulin
- Figure S1: Synthesis and characterization of the macrocycle (15)aneN4 S; Table S1: Mean ± Standard Error for each
Methods (brief)
- was performed by NMR, FT-IR spectroscopy and electrospray ionization mass spectrometry (ESI-MS)
- cocktail (Roche; 1 tablet per 10 mL)). Before activity determinations, samples were centrifuged at
- sample with 5× diluted Coomassie dye (Bio-Rad) in 96-well plates, followed by measurement of
- Holmgren (27). For TrxR activity, samples (40 µg of protein) were incubated in 96-well plates with
- same procedure used for TrxR, but with the samples being incubated with 100 nM recombinant rat
- levels of TrxR and Trx. Samples (30 µg of protein) were separated under reducing conditions with
-
- Bjørklund, G.; Mutter, J.; Aaseth, J. Metal chelators and neurotoxicity: Lead, mercury, and arsenic. Arch. Toxicol.
-
- Cao, Y.; Skaug, M.A.; Andersen, O.; Aaseth, J. Chelation therapy in intoxications with mercury, lead and
-
- Bjørklund, G.; Crisponi, G.; Nurchi, V.M.; Cappai, R.; Djordjevic, A.B.; Aaseth, J. A Review on Coordination
Implications
This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.
Wiki pages this source may touch
- Fish — marine, predatory (tuna, swordfish, shark, king mackerel)
- Shellfish (shrimp, crab, lobster, clams, oysters, mussels)
- Mercury
- Mercury
- Cadmium
- Arsenic
- Nickel
Verification notes
- Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing
wiki/sources/pages before creation. - Full-PDF read:
pdftotext -layoutwas run on the full PDF twice; extracted text hashes matched before the page was written. - Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
- Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
- HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.
Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.