Overview
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Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- effects including acceleration of healing and strengthening of gen flow and 5% Isoflurane (Baxter; HDG9623). Once asleep,
- In the whole organism, as well as in single cells, was diminished to 3%. The dorsal area was shaved and
- two or more of these metals can be competitors and bind to the subcutaneously (200 mg/kg of body weight) pre-operatively.
- Our study focuses on four enzyme-associated elements scab. Three of the samples were fixed in 4% paraformalde-
- (Table 1). undergoing reduction mammoplasty was approved by the eth-
- animal protection act and approved by the Institutional After 7 days of fixation in 4% paraformaldehyde phosphate-
- pathogen-free condition with 12-h day-night cycle, with food concentrations from 50% to 99.9% (V/V), then with pure xylol
- nine time-groups (n = 3): 8 and 16 h and 1, 2, 3, 5, 7, 10, 39603002). Samples were completely cut with a microtome
- Table 1 Evolution of the kinetics in phases of wound healing. Results are represented as constant (0), decrease (−), or increase (+) in comparison to the
- mined as the median of the slices regarding the wound mar- temperature for 90 min, rinsed with TBS, and incubated with
- taining 0.3% Triton-× 100 (Sigma; 9002-93-1) for 5 min. accelerated reaction system (CEM, Mars 5). Each sample
- 2% of bovine serum albumin (BSA) for 1 h. The primary mixed with 1.3 ml nitric acid (65%, Suprapure, Merck) and
- with 1% BSA, was incubated overnight at 4 °C in a wet cham- completely into a 5-ml volumetric glass flask and brought
- with 1% HNO3 (Suprapure, Merck) was set to 10 s to avoid
- dard solution, consisting of 1% HNO3 containing 10 μg/l 6-μm thin cut of wounds taken at the median part of the wound ± SD (n =
- yttrium and 10 μg/l thulium. A calibration was carried out
- concentrations of copper and zinc varying from 0 to 10 ppb
- rat’s values were the average of its wounds values (n = 3 for
- significance determination at a 95% confidence level. We used
- were measured. Despite of the use of a 6-mm diameter biopsy H&E staining of 6-μm thin cut of wounds taken at the median part of
- punch for the wounding, the embedding and microtomic pro- the wound ± SD (n = 3 rats in each group; one rats data is the mean of 3
- staining of 6-μm thin cut of wounds taken at the median part of the wound
- 3.1 mg) in order to set the wounds (Table 2). We also per-
- mammoplasty (mean dry weight of the samples 33.0 ±
- 0.02). All data were displayed as mean ± SD of three rats (n = 3 rats in pathogens, and starting debridement of the wound bed. (1). As
- each group; one rat’s data is the mean of 3 wounds) skin lesions trigger wound healing, we assume the
- Table 2 Concentration baselines in rat and human healthy skin. ICP-MS results of the mean of at least 3 samples ± SD. * (16)
- baseline. All data were displayed as mean ± SD of three rats (n = 3 rats baseline. All data were displayed as mean ± SD of three rats (n = 3 rats
- experiment in comparison with healthy skin baseline (Table 2 This increased concentration can be attributed to increased cell
- of copper concentrations (Table 2 and Fig. 8) may result radically. In comparison to the healthy skin base, after
- reach this level within 2 days and remained stable along the In summary, we were able to describe two metal concen-
- eration phase occurred from day 1 to 7, so did the granu- It is notable that, apart from magnesium, none of the metals
- baseline. All data were displayed as mean ± SD of three rats (n = 3 rats should be considered from the late inflammation until the
- in each group; one rat’s data is the mean of 3 wounds) mid-proliferation phase. Copper supplementation, on the
Methods (brief)
- of enzymatic or other protein activities and ion availability. and preserved as control sample. Post-operative analgesia was
- Our study focuses on four enzyme-associated elements scab. Three of the samples were fixed in 4% paraformalde-
- scribe the kinetics of these elements in the phases of natural trometry (ICP-MS) measurements.
- written consent for tissue donation. Human skin samples were
- Committee on Use and Care of Animals of Hanover buffered saline solution (0.1 M; pH = 7.4), samples were
- 12/0993). Twenty-seven male Lewis rats (Charles River; previously described protocol (14). The samples were impreg-
- nine time-groups (n = 3): 8 and 16 h and 1, 2, 3, 5, 7, 10, 39603002). Samples were completely cut with a microtome
- water, samples were stained 5 min in hematoxylin (Roth; After washing, slides were covered with Vectashield
- were deparaffinized and rehydrated as described above. After Inductively Coupled Plasma Mass Spectrometry
- taining 0.3% Triton-× 100 (Sigma; 9002-93-1) for 5 min. accelerated reaction system (CEM, Mars 5). Each sample
- monoclonal antibody; RM-9106-S0) 1:100 diluted in TBS digestion at 600 W, each sample solution was poured
- ber. After rinsing with TBS, a biotinylated secondary antibody up to volume using double distilled water. The samples were
- Inductively coupled plasma mass spectrometry (ICP-MS)
- measurements were carried out using a quadrupole ICP-MS
- system (Perkin Elmer, Elan 6000) with an auto sampler sys-
- lizer gas flow, and a sample flow rate of 1 ml/min regulated by
- (ICP multielement standard IV solution, Merck) once every Fig. 1 Histologic measurement of wound diameter development. Data
- 10 samples. Samples were diluted 1:10 with an internal stan- were calculated by measurements of wound margins of H&E staining of
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Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.