BREEDING, SEED PRODUCTION AND PLANT BIOTECHNOLOGY

Overview

This source page is a mechanical bulk-ingest record for a PDF in the methylmercury infant-formula research pull. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.

Key numbers

The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:

• логических методов. Овощи России. 2025;(6):5- Brassicaceae Burn. (редис, дайкон, репа). Сроки создания сортов и гибридов корнеплодных куль-
• 14, Selektsionnaya str., VNIISSOK,
• (Root Crops and Tubers – 2025).” The authors up to 25 embryoids has been achieved, and for carrot, up to 55 embryoids per 100 in vitro unpollinated
• committee for including this work in the conference Brassicaceae family for radishes, the maximum efficiency of the technology was 8 embryoids per Petri
• dish, 53 embryoids for turnips, 161 embryoids for carrots, and 20 embryoids for parsnips. Interspecific
• “Vegetable crops of Russia” since 2023, but had were obtained using the “embryo rescue” technology. The method of clonal micropropagation has been
• flicts of interest. (Zhegalov, Venya, Persey), and obtaining a patent for invention No. 2807444 “Microspore isolation
• 2025;(6):5-17. (In Russ.) logical methods into the breeding process with the close cooperation of biotechnologists and breeders.
• Received: 22.10.2025 acceleration of breeding, root crops, biotechnological methods, in vitro culture, doubled haploids (DH-
• Accepted for publication: 18.11.2025 lines), clonal micropropagation, “rescue” of embryos, culture of isolated microspores, culture of unpolli-
• I S S N 2 6 1 8 - 7 1 3 2 ( On lin e ) Ово щ и Ро ссии №6 2025 ( 5 ) Vegetable crops of Russia №6 2025 ISSN 2072-9146 (Print)
• I S S N 2 6 1 8 - 7 1 3 2 ( On lin e ) Ово щ и Ро ссии №6 2025 ( 6 ) Vegetable crops of Russia №6 2025 ISSN 2072-9146 (Print)
• Использовали специальные плашки 6-well Culture Plate (Carl Zeiss Microscopy GmbH, Germany) при увеличении 10х.
• (Corning, USA) с добавлением 2 мл жидкой питательной Чашки Петри с семяпочками культивировали при посто-
• среды MSm и 12-well Culture Plate (Corning, USA) с добавле- янном покачивании 60 об/мин на шейкере при температуре
• семейства Brassicaceae (11). на Западно-Сибирской овощной опытной станции –
• I S S N 2 6 1 8 - 7 1 3 2 ( On lin e ) Ово щ и Ро ссии №6 2025 ( 7 ) Vegetable crops of Russia №6 2025 ISSN 2072-9146 (Print)
• the induction MSm nutrient medium supplemented with 0.2 mg/L 2,4-D and 0.2 mg/L kinetin after 2 weeks of cultivation (A); a bud
• with ovules that have ruptured through the ovary wall after 3 weeks of cultivation on MSm medium with 0.2 mg/L 2,4-D and 0.2
• mg/L kinetin (Б); a Petri dish with browned isolated ovules at 6 weeks of cultivation, with three ovules showing formation of
• I S S N 2 6 1 8 - 7 1 3 2 ( On lin e ) Ово щ и Ро ссии №6 2025 ( 8 ) Vegetable crops of Russia №6 2025 ISSN 2072-9146 (Print)
• Fig. 2. Stages of the technology for producing carrot (Daucus carota L.) plants via microspore culture: multicellular embryoid
• I S S N 2 6 1 8 - 7 1 3 2 ( On lin e ) Ово щ и Ро ссии №6 2025 ( 9 ) Vegetable crops of Russia №6 2025 ISSN 2072-9146 (Print)
• раз у каждого из двух сортообразцов (18) (Romanova et al., ся уже независимо. Использование платформы-шейкер
• Table 1. Comparison of carrot embryoid yield from genotypes No. 7 and No. 12 of the breeding line «Altayskaya Lakomka»
• Fig. 4. Embryoids proliferated on MSm nutrient medium with 2% sucrose supplemented with 0.2 mg/L kinetin: primary embryoid
• I S S N 2 6 1 8 - 7 1 3 2 ( On lin e ) Ово щ и Ро ссии №6 2025 ( 10 ) Vegetable crops of Russia №6 2025 ISSN 2072-9146 (Print)
• Fig. 5. Stages of the technology for producing parsnip (Pastinaca sativa L.) plants via microspore culture:
• I S S N 2 6 1 8 - 7 1 3 2 ( On lin e ) Ово щ и Ро ссии №6 2025 ( 11 ) Vegetable crops of Russia №6 2025 ISSN 2072-9146 (Print)
• Fig. 6. Development of a technology for producing gynogenic plants of table beet (Beta vulgaris L.) in unfertilized ovule culture in
• with 0.4 mg/L TDZ (A); formation of a gynogenic structure resulting from the rupture of the micropylar part of the ovule after 4-6
• семяпочки, определялась генотипом и варьировала от 0 до (n=x=9) (5).
• I S S N 2 6 1 8 - 7 1 3 2 ( On lin e ) Ово щ и Ро ссии №6 2025 ( 12 ) Vegetable crops of Russia №6 2025 ISSN 2072-9146 (Print)
• бутонами. Использование в качестве исходных эксплантов танный для культур семейства Brassicaceae (11). Этот тех-
• I S S N 2 6 1 8 - 7 1 3 2 ( On lin e ) Ово щ и Ро ссии №6 2025 ( 13 ) Vegetable crops of Russia №6 2025 ISSN 2072-9146 (Print)
• Fig. 9. A DH-line of European radish «Zhegalov»
• I S S N 2 6 1 8 - 7 1 3 2 ( On lin e ) Ово щ и Ро ссии №6 2025 ( 14 ) Vegetable crops of Russia №6 2025 ISSN 2072-9146 (Print)
• I S S N 2 6 1 8 - 7 1 3 2 ( On lin e ) Ово щ и Ро ссии №6 2025 ( 15 ) Vegetable crops of Russia №6 2025 ISSN 2072-9146 (Print)
• 2. Федорова М.И., Степанов В.А. Корнеплодные овощные растения, Akhramenko V., et al. Embryogenesis induction of carrot (Daucus carota L.)
• направления селекции, результаты. Овощи России. 2017;(4):16–22. in isolated microspore culture. Foods and Raw Materials. 2023;11(1):25–34.
• 3. Домблидес А.С. Разработка лабораторной технологии получения Haploid Embryo Genome Doubling with Colchicine and Trifluralin.
• 4. Masuda K., Kikuta Y., Okazawa Y. A revision of the medium for 20. Fujimura T., Komamine A. Synchronization of Somatic Embryogenesis

Methods (brief)

• Analytical method details were not mechanically resolved from extracted text.

Implications

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Wiki pages this source may touch

• Fish — marine, non-predatory (sardines, anchovies, salmon, cod)
• Aluminum

Verification notes

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Update history

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