Overview
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Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- poisoned fatty liver patients. The accuracy of distinguishing this disease via decreased PC molecules was 83.33%.
- at 32.4% (1, 2). In 2023, an international multisociety MASLD (5, 6). Among them, arsenic is a widely present
- arsenic levels exceeding 10 μg/L, which is above the rec- specific enzymes and proteins, integral to lipid trans-
- future use. Morning urine samples were collected, and Mixture F-12 (DMEM/F12) medium containing 10% fetal
- the urinary arsenic load was measured. A urine sample of bovine serum, 1% insulin-transferrin-selenium (ITS),
- 1 mL was digested with 5 mL of concentrated nitric acid and 1% dexamethasone at 37 °C with 5% carbon diox-
- and diluted to 10 mL with 1% nitric acid, and then the ide. AML12 cells were treated with NaAsO2 at different
- coupled plasma mass spectrometry (ICP-MS) (Thermo apparent cytotoxicity (cell viability > 80%) was observed
- and 10.0 mg/L, respectively (14), with a standard diet roform–methanol (2:1, v/v) containing mixed internal
- animals were anesthetized with 1% sodium pentobarbi- is detailed in Additional file 1: Table S1. After ultra-
- 4% paraformaldehyde for fixation, followed by section- natant was collected for analysis. Samples were analyzed
- with Q Exactive mass spectrometer (Thermo Fisher follows: precursor/product tolerance 5 ppm and prod-
- Scientific, USA) on an ACQUITY UPLC BEH C8 Col- uct ion threshold 5%. Finally, the relative abundance of
- 32% B equilibration (1.5 min), linear increase to 85% B sample possessed the same volume as the individual
- (14 min), ramp to 97% B (0.1 min), hold (2.4 min), return samples. Analysis of the QC data indicates a satisfactory
- analyzed in data-dependent MS/MS mode (m/z 150– Liver tissues fixed in 4% paraformaldehyde were pro-
- identified lipids are detailed in Additional file 1: Table S2. quently cultured and treated using the methodology out-
- lipid standard mixture (Additional file 1: Table S3), 800- to stain lipids within liver tissue or AML12 cells, render-
- follows: 30% B for 2 min, linear increase to 100% B over the utilization of a reaction involving glycerol-3-phos-
- 23 min, and then a quick drop to 30% B, which is main- phate oxidase and peroxidase, while the VLDL content
- 50%. Samples were analyzed in data-dependent MS/MS tively. Liver tissues or AML12 cells (1 × 106) were rapidly
- Additional file 1: Table S4. uct of PC or choline in the supernatant and a fluorescent
- file 1: Table S5. The levels of mRNA expression of genes between two groups were analyzed using either an inde-
- in Additional file 1: Table S6. Then, the PCR amplification
- Table 1 General demographic information
- Variable Arsenic-poisoned fatty liver patients (n = 30) Control group (n = 30) p-value
- Male 11 (36.70%) 11 (36.70%) 0.605b
- Female 19 (63.30%) 19 (63.30%)
- Urine arsenic (μg/L, x ± s) 26.29 ± 10.85 14.94 ± 4.73 < 0.001c
- Normal 0 (0.00%) 7 (23.30%) 0.011b
- Abnormal 30 (100.00%) 23 (76.70%)
- Normal 1 (3.30%) 21 (70.00%) < 0.001b
- Abnormal 29 (96.70%) 9 (30.00%)
- Normal 25 (83.30%) 27 (90.00%) 0.706b
- Abnormal 5 (16.70%) 3 (10.00%)
- Normal 25 (83.30%) 26 (86.70%) 1.000b
- Abnormal 5 (16.70%) 4 (13.30%)
- Normal 25 (83.30%) 27 (90.00%) 0.706b
- Abnormal 5 (16.70%) 3 (10.00%)
- Normal 22 (73.30%) 28 (93.30%) 0.038b
- Abnormal 8 (26.70%) 2 (6.70%)
- such as age, gender, smoking status, and BMI (Table 1). tion (Fig. 1D and Additional file 1: Table S2). We further
Methods (brief)
- tally relevant doses of sodium arsenite (NaAsO₂) was established, with liver lipid metabolomics applied to assess
- with an in vitro model of NaAsO₂-induced lipid accumulation in hepatocytes, methods including RT-qPCR, West-
- mentioned arsenic-poisoned fatty liver patients. All par- liver tissue was conducted using ICP-MS on the digested
- ticipants were permanent residents of the local area. The and deacidified liver samples, with the results standard-
- bolic deficiency. Fasting blood samples were collected, Mouse hepatocyte AML12 (Procell, Wuhan, China) was
- future use. Morning urine samples were collected, and Mixture F-12 (DMEM/F12) medium containing 10% fetal
- the urinary arsenic load was measured. A urine sample of bovine serum, 1% insulin-transferrin-selenium (ITS),
- and diluted to 10 mL with 1% nitric acid, and then the ide. AML12 cells were treated with NaAsO2 at different
- coupled plasma mass spectrometry (ICP-MS) (Thermo apparent cytotoxicity (cell viability > 80%) was observed
- study. were treated with 5 µmol/L NaAsO2 for 24 h, accom-
- sus, all previously diagnosed NAFLD cases automatically NaAsO₂ for an additional 24 h.
- standards. Serum samples from both human subjects and mice were
- collected. The levels of hepatic function markers alanine
- istered aqueous solutions of sodium arsenite (NaAsO2) Lipidomics
- was collected from the abdominal aorta, and the serum centrifugation (13,000 rpm, 4 °C, 10 min), the lower
- 4% paraformaldehyde for fixation, followed by section- natant was collected for analysis. Samples were analyzed
- ammonium acetate. A 20-min gradient elution was (QC) samples were prepared by mixing equal volumes
- applied (flow rate 0.26 mL/min, injection volume 3 μL): of extracts from all the samples, ensuring that each QC
Implications
This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.
Wiki pages this source may touch
- Fish — marine, non-predatory (sardines, anchovies, salmon, cod)
- Shellfish (shrimp, crab, lobster, clams, oysters, mussels)
- Arsenic
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Update history
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