G-triplex DNA biosensor for mercury detection validated in milk

Overview

A biosensor based on a G-triplex DNA structure was developed for Hg2+ detection and validated in spiked milk samples. The sensor uses the T-Hg2+-T coordination chemistry that stabilises the G-triplex structure as the Hg2+ recognition mechanism. No unspiked occurrence measurements of Hg in milk are reported; validation is by spike-recovery in milk matrix.

Key numbers

• LOD, linear range, and recovery in spiked milk in full paper.
• No occurrence data for Hg in milk; all milk tests are spiked.

Methods (brief)

G-triplex DNA biosensor exploiting T-Hg2+-T coordination. Measures total inorganic Hg2+; does not distinguish methylmercury. Milk matrix spike-recovery validation.

Implications

Certification: Method development; milk matrix validation demonstrates analytical feasibility but provides no baseline Hg occurrence data for dairy. Courses: Demonstrates G-triplex DNA biosensor design exploiting specific Hg-DNA coordination chemistry.

Wiki pages updated on ingest

• Mercury
• milk

Update history

The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.