Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- a decreased thioredoxin reductase (TrxR) activity was found in the liver (40%) and the brain
- (5 min and 18 h) resulting in the formation of several metal-peptide adducts (see Table S1).
- The results of the above-described experiments are summarized in Table 1, which shows the
- mixture did not change significantly after 18 h of incubation (Table 1 entry n°2 and Figure 3).
- seen in Table 1 (entry 2) and Figure S4: the mono-metalated adduct was completely
- Table 1. Reactivity of TrxR(488-499) incubated with MeHgCl (0.5 or 3 MeHg+) for 5 min or 18 h, at pH 7.0 and 37 °C, after a 30 min. reduction step of the peptide with 10
- S1 and Table S1) as well as previous literature reports describing the reduction of disulfide
- thermodynamically favorable (Table S2). The reaction is strongly exergonic with MeHgCl (ΔG
- is the most stable one, while the S-bound isomers 4’’ (with Se→Hg interaction) and 4’’’
- equiv. of MeHgCl for a 5 min in water solution (Table 1 and Figures S13 and S14).This is the
- from the mono-metalated adduct, while the C-H bond forms partially (27.9 % elongated from
- peptide-adducts (see Table 1). The above calculations revealed that the Hg-bridged complex
- were observed, covering 97% of the GPx1 sequence. Of these peptides, those containing Cys80,
Methods (brief)
- chromatography (LC) coupled to tandem electrospray mass spectrometry (ESI-MS) was
- First, Carvalho et al. 14 used mass spectrometry to demonstrate that reduced form of
- chromatography (LC) coupled to tandem electrospray mass spectrometry (MS/MS),
- as a target for mercury and gold in biological systems: Contributions of mass spectrometry and
Implications
This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.
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Verification notes
- Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing
wiki/sources/pages before creation. - Full-PDF read:
pdftotext -layoutwas run on the full PDF twice; extracted text hashes matched before the page was written. - Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
- Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
- HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.
Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.