Mercury Based on DNA-Templated Gold Nanoclusters

Overview

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Key numbers

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detect as low as 20.0 nM (4.0 ng Hg/g) and has a good linear detection range, with target
their products to as low as 1.0 mg/kg. However, the most fundamental or challenging task
All the ssDNA sequences (Table S1 in Supplementary Materials) we used in this
containing 10% heated, inactivated FBS. HeLa cells were washed twice with PBS solution
measured, where cell viability (%) = (OD450 of mercury species group—OD450 of blank
fluorescent imaging analysis, and the fluorescent emission range is collected from 400 nm
AuNCs. Moreover, their fluorescence quantum yield (QY) was expected to approach 6%,
formed by reducing mixed Au3+ , Ag+ and Hg2+ . In the presence of OrHg, the mean size of
AuNCs (especially in the AA section of AA-T7 ) to form Ag/AuNCs, and meanwhile, OrHg
AuNCs were unstable and vulnerable to Hg2+ and OrHg attack, thereby affecting their
As mentioned above, AA-T7 was the most suitable scaffold for establishing a stable
pH 7.5 was chosen as the most suitable pH as a result of specific fluorescence quenching by
are well below the maximum mercury level (0.5 mg/kg) for food according to the latest
assay (47,48,63–67) (Table 1).
Table 1. Comparison of the analytical performance of the proposed method with other methods.
As listed in Tables 2 and 3, the content of Hg2+ or OrHg in tap water, seawater and dried
golden pomfret fish muscle samples was accurately determined with Rec. of 98.0–108.0%
and a relative standard deviation (RSD, n = 6) ≤ 5.0%. The above-obtained actual samples
Table 2. Analytical results of Hg2+ in tap water, seawater and dried golden pomfret fish muscle samples.
Table 3. Analytical results of OrHg in tap water, seawater and dried golden pomfret fish muscle samples.
Meanwhile, the introduction of trace Hg2+ or OrHg could effectively induce fluorescence
AuNCs with/without Hg2+ , MeHg or EtHg (n = 4); Figure S11: Intracellular confocal fluorescence
field; (f) Hela cells co-incubated with AA-T7 -templated AuNCs and EtHg at dark field; Table S1: The
1. Galhano, J.; Kurutos, A.; Dobrikov, G.M.; Duarte, M.P.; Santos, H.M.; Capelo-Martínez, J.L.; Lodeiro, C.; Oliveira, E. Fluorescent
nanoclusters on a portable paper-based device. Chem. Eng. J. 2022, 430, 133070. (CrossRef)

Methods (brief)

Hg2+ and OrHg in tap water, seawater and dried golden pomfret fish muscle samples,
samples and aquatic products to ensure human health and food safety.
gas chromatography (GC) and high-performance liquid chromatography (HPLC) with
detection techniques like inductively coupled plasma mass spectrometry (ICP-MS), atomic
fluorescence spectrometry, and atomic absorption spectroscopy (AAS), exhibit high preci-
OrHg in water and seafish samples, thereby providing technical support for improving the
experiment were synthesized and verified through HPLC purification and mass spectrom-
using an Agilent 7800 ICP-MS (Santa Clara, CA, USA). The pH was measured by the Ohaus
2.3.3. Pretreatment and Determination of Actual Samples
Tap water and seawater samples collected from the lab tap and coastal waters of
The selected seafish samples were dried golden pomfret fish muscle collected from
Section 2.3.2, respectively. Tap water, seawater and seafish samples spiked with different
fluorescent imaging analysis, and the fluorescent emission range is collected from 400 nm
collected under the above same procedures and optimal conditions, selected above and the
LOD (µM) LOQ (nM) Detection Range (µM)
3.7. Determination of Hg2+ and OrHg in Actual Samples
this fluorescent sensing method, actual samples were pretreated and analyzed for mercury
a lab tap, seawater and seafish (dried golden pomfret fish muscle) samples collected from

Implications

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Verification notes

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Update history

The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.

Commit	Date	Change	Description
b01ec52c	2026-08-04	major	2 sections added
d49e450f	2026-08-03	major	5 sections added; narrative text revised