Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- pressed cell proliferation, whereas 1 µM Ni showed no detectable adverse effect compared
- (Figure 5C) with a calculated binding energy of −5.76 kcal/mol (Supplementary Table S1),
- (Figure S7, Supplementary Table S1). The analysis identified specific high-affinity binding
- sites within the collagen triple helix, revealing that Ni2+ forms a stable coordination complex
- Ni2+ engagement at Glu-10 may induce a local structural rearrangement that favors ILK
- For tissue sections, samples were fixed overnight in 4% PFA at RT. Antigen retrieval
- subsequently permeabilized with 0.1% Triton X-100 in PBS for 10 min and blocked with
- 5% normal serum (species-matched to secondary antibodies) plus 1% BSA for 1 h at RT.
- Primary antibodies diluted in 1% BSA/0.1% Tween-20/PBS were applied overnight at
- humidified 37 ◦ C, 5% CO2 incubator; residual DCFH-DA was subsequently aspirated, cells
- Table 1. Primers used for RT-qPCR analysis.
- were blocked in 5% non-fat dry milk for 2 h at room temperature, followed by overnight
- dard conditions (37 ◦ C, 5% CO2 ). sh-ILK lentivirus (sc-35667-V, Santa Cruz Biotechnology,
- complete α-MEM (10% FBS, 1% penicillin–streptomycin), and cells were cultured for an
- 4% paraformaldehyde.
- Type 2 Diabetes Mellitus Risk: A Protective Effect within a Specific Range. J. Trace Elem. Med. Biol. 2024, 82, 127362. (CrossRef)
Methods (brief)
- in serum from both mice and humans. In situ LA–ICP–MS further showed that Ni levels in
- tively coupled plasma mass spectrometry (ICP-MS), we demonstrated that Ni content in
- applied in situ laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)
- (D) Schematic of LA-ICP-MS and quantification of CaO and Ni in embryonic skulls. Statistical
- separation between NiCl2 -treated and control samples with widespread differential ex-
- to enhanced downstream AKT activation. While our ICP-MS data conclusively map the
- For tissue sections, samples were fixed overnight in 4% PFA at RT. Antigen retrieval
- Cranium and femur samples were decalcified using a commercial ethylenediaminete-
- Serum samples were digested in concentrated HNO3 and H2 O2 using a microwave
- digestion system. The total Ni concentration was subsequently quantified using Inductively
- Coupled Plasma Mass Spectrometry (ICP-MS, Agilent 7800, Santa Clara, CA, USA), utilizing
- mass spectrometry (LA-ICP-MS) was directly performed to determine the metal contents
Implications
This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.
Wiki pages this source may touch
- Fish — marine, predatory (tuna, swordfish, shark, king mackerel)
- Fish — marine, non-predatory (sardines, anchovies, salmon, cod)
- Nickel
- Aluminum
- Chromium
Verification notes
- Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing
wiki/sources/pages before creation. - Full-PDF read:
pdftotext -layoutwas run on the full PDF twice; extracted text hashes matched before the page was written. - Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
- Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
- HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.
Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.