Overview
This source page is a mechanical bulk-ingest record for a PDF in the methylmercury infant-formula research pull. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
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- Ibα-XYL1 Interfered Expression Decreases Starch Granule Size
- 1 Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Key Laboratory of Biology and Genetic
- Breeding of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou 221131, China;
- lichen@jaas.ac.cn (C.L.); 20081003@jaas.ac.cn (M.K.); yclucky2022@126.com (C.Y.);
- xzsongweihan@163.com (W.S.); 20091002@jaas.ac.cn (W.T.); yanhuijsxz@jaas.ac.cn (H.Y.);
- grf15852141027@gmail.com (R.G.); xznkywx@163.com (X.W.); zhangyungang@jaas.ac.cn (Y.Z.)
- 2 School of Life Science, Jiangsu Normal University, Xuzhou 221116, China; brave_ma_2022@163.com
-
- Correspondence: liqiang@jaas.ac.cn; Tel.: +86-0516-8218-9203
- sweetness and taste. A xylosidase gene (Ibα-XYL1) was cloned from sweetpotato variety
- at cell membrane. Reduction in Ibα-XYL1 gene expression had no significant effect on
- Received: 15 March 2025 starch pasting temperature, promote the conversion of starch to maltose, increase the
- Revised: 14 May 2025 soluble sugar content, and improve the sweetness and taste of steamed transgenic SPSR.
- R.; Wang, X.; Zhang, Y.; et al. Ibα-XYL1
- Mol. Sci. 2025, 26, 5015. https:// cultural crop in China. Its stem and leaf can be eaten as vegetable, and its storage root can
- Copyright: © 2025 by the authors. varieties can be used as landscaping material. Due to its plentiful uses, it is widely planted
- and has antioxidant activity and is highly popular with people (1). However, due to the
- licenses/by/4.0/). contains four soluble sugars: glucose, fructose, sucrose, and maltose (2). The soluble
- produced by starch gelatinization in the ripening process (3). The starch gelatinization
- to gelatinize and decompose, so they have a lower gelatinization temperature (4). Related
- produced (5). Overexpression of sucrose non-fermentation-associated protein kinase-1
- (SnRK1) can increase starch content, decrease amylose ratio, increase granule size, and
- increase crystallinity and gelatinization of transgenic sweetpotato (6), but there are no
- In addition, maltose content is also affected by amylase, especially β-amylase (7,8).
- maltose (5). However, when maltose content increases to a certain amount, it will no longer
- as substrate restriction and feedback of high concentration products (9). Since β-amylase
- reports found that vacuolar invertases (IbINV) (10) and fructose-1,6-bisphosphate aldolase
- (IbFBA5) (11) can increase SPSR soluble sugar content, but whether it affects SPSR taste has
- Alpha-xylosidase (α-XYL) is a member of the glycoside hydrolase 31 family. Like the
- was discovered in some bacteria, such as Sulfolobus solfataricus (12), Cellvibrio japonicus (13),
- Aspergillus niger (14), etc. It is the first report that α-xylosidase-coding proteins have
- hydrolyzes the α-glucoside bond in rice (15). However, in Arabidopsis thaliana, α-xylosidase
- germination (16), and is also involved in polysaccharides decomposition, especially xylo-
- oligosaccharides (17). When AtXYL1 was introduced in a suitable expression vector and
- cells, although to a lesser extent (18). Arabidopsis mutant seeds lacking TRG1/XYL1 showed
- and decreased free xylooligosaccharides content (16). However, the function of xylosidase
- 2.1. Cloning, Sequence and Expression Pattern Analysis of Ibα-XYL1
- data (19) was compared with sweetpotato GARDEN (http://sweetpotato-garden.kazusa.
- or.jp/index.html). The SPSR cDNA of variety ‘Yanshu 25’ was used as a template to am-
- plify the target gene fragment (Figure 1a). After sequencing and alignment, the unigene
- was annotated as a xylosidase encoding gene, which was homologous to Itfα-XYL1, and
- was therefore named Ibα-XYL1. The Itfα-XYL1 sequence comes from a public database
- Figure 1. Cloning, sequence and expression pattern analysis of Ibα-XYL1. (a) The gel map of Ibα-XYL1
Methods (brief)
- lichen@jaas.ac.cn (C.L.); 20081003@jaas.ac.cn (M.K.); yclucky2022@126.com (C.Y.);
- xzsongweihan@163.com (W.S.); 20091002@jaas.ac.cn (W.T.); yanhuijsxz@jaas.ac.cn (H.Y.);
- grf15852141027@gmail.com (R.G.); xznkywx@163.com (X.W.); zhangyungang@jaas.ac.cn (Y.Z.)
-
- Correspondence: liqiang@jaas.ac.cn; Tel.: +86-0516-8218-9203
- Each community planted 20 plants and set up 3 replicates. Samples were taken 130 days
- Different tissue samples were derived from ‘Yanshu 25’ plants at 130 days after planting
- was digested by double NcoI and SwaI digestion. The FS-Ibα-XYL1, RS-Ibα-XYL1, and
- The pFGC5941 digestion product, FS-Ibα-XYL1, RS-Ibα-XYL1, and intron fragments were
- to measure the quality indicators of steamed sweetpotato. Fresh samples were sampled,
- dryer for freeze-drying. The other group of samples was placed in a steaming box, steamed
- liquid chromatography (HPLC, PAL-1, Guangzhou Atago Scientific Instrument Co., LTD,
- distilled water and the filtrate was then collected. The supernatant was removed after
- after 4 min; the whole process lasted 16.7 min. Three replicates were taken for each sample.
- storage roots were steamed, and the samples were randomly numbered. Samples were
- mouth with clean water after evaluating each sample and then all samples were evaluated
- in turn. Samples were scored for sweetness, fiber content, taste, and total score. Finally, the
-
- Wang, H.; Feng, Y.; Guo, K.; Shi, L.; Xu, X.; Wei, C. Structural, thermal, pasting and digestion properties of starches from
- cellulose inhibition of α-amylase and alpha-glucosidase in simulated digestion of starch. Food Funct. 2020, 11, 4719–4731.
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