phylum “Candidatus Pyropristinus” was proposed for the MAG sequence of “Candida­

Overview

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Key numbers

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• observed at oxygen concentrations between 2% and 10%(v/v), indicating that the strain
• presence of 5%(v/v) oxygen as an electron acceptor, growth was not observed in the
• the presence of 5%(v/v) oxygen at 70°C, the good growth of strain sy37 was observed
• a G + C content of 60.6 mol%. Genome annotation with DFAST analysis revealed that the
• (89.1%) obtained from Juan de Fuca Ridge flank fluids (7, 32). The environmental
• Basin, were highly related to strain sy37 with sequence similarities of 86.5%, 86.5%,
• 85.3%, and 86.4%, respectively. The representatives of phylum “Ca. Caldipriscota,” “Ca.
• WOR-3 lineage (Table S1), indicating that the WOR-3 lineage was a monophyletic lineage
• accession numbers are shown. The numbers are indicated at the nodes when above 80% bootstrap value was obtained (neighbor-joining method/maximum-
• for sulfur reduction (34, 35). The SudA-like protein of strain sy37 was 40.2% sequence
• The accession numbers used for the analysis are shown in Table S1. Bar, 0.5 substitutions per amino acid site.
• completeness and <10% contamination values by CheckM; Table S1), the phyloge­
• for the analysis are shown in Table S1. Bootstrap probabilities are indicated at branching points. Solid squares (red), open squares (orange), solid circles (dark
• were allowed to form for 4 days on medium solidified with 0.6% (w/v) gellan gum in vials.
• were fixed with 2% glutaraldehyde, postfixed with 1% osmium tetroxide, and dehydrated
• electron microscopy observation, the cells were fixed with 2.5% glutaraldehyde and then
• H2, O2, CO2, 0.5%(w/v) elemental sulfur, 10 mM nitrate and 10 mM acetate.
• 5 mM selenite, 5 mM arsenate, 5 mM ferric citrate, 0.1%(w/v) ferrihydrite (49), and 5%(v/v)
• used for the analysis of PhyloPhlAn (Table S1). iTOL was used to visualize the phyloge­
• Supplemental material (AEM00188-25-s0001.pdf). Table S1; Fig. S1 and S2.

Methods (brief)

• of samples from various environments. The isolated strain was a thermophilic, microaer­
• gene sequences in samples recovered from various environments, have come to have
• fluid sample from Juan de Fuca Ridge (7).
• collected from public databases, and the phylogenetic topology of the WOR-3 lineage,
• A novel thermophilic bacterium was isolated from a thermal fluid collected from a
• Sample collection, enrichment, and isolation
• The hydrothermal fluid for enrichment and isolation was collected from a deep-sea
• measured temperature of fluid was approximately 70°C. The fluid was collected in a
• cooperation with samples collection. We thank Shinobu Iwasaki and Keiko Nanri for
• BioSample accession number SAMD00856355.
• 12. Colman DR, Jay ZJ, Inskeep WP, Jennings R deM, Maas KR, Rusch DB, Woyke T, Ryan MC, Banfield JF. 2018. Differential depth distribution of
• https://doi.org/10.1128/jb.176.21.6509-6517.1994 archaeon isolated from a deep-sea rock collected at Suiyo Seamount,

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Wiki pages this source may touch

• Fish — marine, non-predatory (sardines, anchovies, salmon, cod)
• Aluminum

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