nutrients in commercial fish species

Overview

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Key numbers

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• n = 30; herring: n = 19). All fish were collected using trawls. Fish were weighed, measured
• digest. The digest was diluted to 10 % acid by weight with ultra-pure water and submitted
• ICP-MS. Recoveries of Hg in standard reference materials were 95 % ± 9 (mean ± standard
• deviation) for DORM-4 Fish Protein (n = 17) (National Research Council of Canada),
• 104 % ± 12 for TORT-3 Lobster Hepatopancreas (n = 12) (National Research Council of
• Canada), and 119 % ± 31 for NIST 2976 mussel (n = 6) (National Institute of Standards and
• Technology). Percent recovery for Se was 98 % ±9 for DORM 4 (n = 17), and 92 % ±6 for
• TORT-3 (n = 12), and 103 % ± 3 for NIST mussel (n = 6). Eighteen duplicate samples were
• run, with relative percent difference (RPD) of 10 ± 8 % for total Hg and 6 ± 6 % for Se.
• 2011, 2008). Percent recovery for DORM-4 was 88 % (n = 2), spike recovery average was
• 97 % (n = 2) and average relative percent difference for duplicates was 1 % (n = 2).
• δ15N analysis were prepared in tin capsules and sent to the UC Davis Stable Isotope Facility
• For herring and dogfish, which have a high lipid content ( > 15 % ; C: N > 4), δ13Clipid free was
• et al., 1992, 1995, Budge et al. 2022). Individual FA’s were evaluated as the % of total
• FA; those with a mean value <0.1 % were removed from the dataset, and the remainder
• processing the tissue for trace metal analyses (mean = 0.2). Additionally, all concentration
• Concentrations of Hg, Se and total lipids were first explored across species (Fig. 2, Table
• 1S). As expected, concentrations of MeHg in a subset of samples (n = 36; Table 2S) were
• combined (Fig. 1S). Concentrations of Hg in these fish were mostly below the 0.3 ppm EPA
• criterion (U.S. EPA, 2001), with only the largest dogfish (n = 17; 35 % of samples), as well
• as the largest cod (n = 1) and skate (n = 2) exceeding it. It is noted that consumption is
• the highest Hg levels (Fig. 2S, Table 1S).
• FA composition (n = 36; Table 3S), a positive relationship was observed between total lipid
• and within species (Tables 1S, 4S). Values of δ15N are an indicator of trophic position
• small range in δ13C across species in this study reflect a similar, phytoplankton base to the
• pelagic) and length explained 73 % of the variation in fish Hg concentration (p < 0.0001,
• Table 5S) across all species, with cod and dogfish, the largest species, having the highest
• by the narrow range of δ13C ratios; this was attributed to a similar planktonic base to the
• across all species (p < 0.0001, Table 5S). The low variance of Se across species has been
• concentrations was explored for each species (Fig. 5; Table 6S), to better understand the
• Table 6S) may have had some influence on their higher least-squares mean Hg concentration
• (Harding et al., 2018)) than those in this study (offshore: −19.1 ± 0.7, n = 19; inshore: −20.0
• ± 0.7, n = 16). However, a study of herring across the continental shelf of the NW Atlantic
• enriched δ13C ratios (−18.5 ± 0.6, n = 126). In that study, δ13C ratios varied by season
• study of the Gulf of Maine (St. Gelais and Costa-Pierce, 2016), but in the same range as
• 2025). Information presented here on Hg and nutrients measured in fish across a range of
• marine fish: inference from stable isotope and fatty acid analyses. Sci. Total Environ 573, 83–95.
• McClelland JW, Valiela I, Michener RH, 1997. Nitrogen-stable isotope signatures in estuarine food
• bay tidal creek system using stable isotopes of carbon and sulfur. Estuaries 20, 77–85.
• Vizzini S, Mazzola A, 2006. The effects of anthropogenic organic matter inputs on stable carbon and
• Ẑvab Rožič P, Dolenec T, Lojen S, Kniewald G, Dolenec M, 2014. Using stable nitrogen isotopes
• analysis. Least squares models are provided in Supplemental Table 5S.

Methods (brief)

• 2.1. Sample collection & processing
• Fish were collected with assistance from Capt. David Goethel of the Ellen Diane, the Yankee
• categorized catch location as either “inshore” or “offshore”, with inshore fish collected in
• shallower waters (<50 m) west of Jeffrey’s Ledge, whereas offshore fish were collected in
• Inshore cod (n = 17) and skates (n = 29) were collected 26 May 2018, inshore red hake (n
• = 32) and silver hake (n = 33), dogfish (n = 20), and herring (n = 16) were collected 10
• July 2018, and offshore fish of all species were collected 12 Oct through 5 Nov 2018 from
• n = 30; herring: n = 19). All fish were collected using trawls. Fish were weighed, measured
• collection. Offshore fish collected during the NMFS fall bottom trawl survey were frozen
• subsamples of the right fillet from the upper anterior portion (skin off) were taken for total
• Samples of individual fish were analyzed, with the exception of very small individuals,
• 2.2.1. Metals: Samples intended for total metal analysis were further processed in a trace
• ICP-MS. Recoveries of Hg in standard reference materials were 95 % ± 9 (mean ± standard
• TORT-3 (n = 12), and 103 % ± 3 for NIST mussel (n = 6). Eighteen duplicate samples were
• the Dartmouth TEA Core by species-specific isotope dilution ICP-MS using a MERX-M
• automated methylmercury analyzer coupled to an ICP-MS (Agilent 7900) (Taylor et al.,
• 2.2.2. Stable isotopes (SI): Freeze dried, homogenous tissue samples for δ13C and
• δ X( %0 ) = (( Rsample∕Rstandard ) − 1) x 1000, where X is either 13C or 15N and R is the ratio of

Implications

This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.

Wiki pages this source may touch

• Fish — marine, predatory (tuna, swordfish, shark, king mackerel)
• Fish — marine, non-predatory (sardines, anchovies, salmon, cod)
• Shellfish (shrimp, crab, lobster, clams, oysters, mussels)
• Mercury
• Mercury
• Tin

Verification notes

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• Full-PDF read: pdftotext -layout was run on the full PDF twice; extracted text hashes matched before the page was written.
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• Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
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Update history

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