Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- was constructed with a substitution mutation of Sec498Cys, enabling 100% expression of the full-length form
- the EF1α promoter, which are functional for stable expression of transcription factors against gene silencing,
- Sec mutant was approximately 5–8% in vitro and 6–10 times lower in transfected cells47. They also highlighted
- the highly effective (~ 50%) processive incorporation of Sec in SelP. However, our results confirmed that total Sec
- lysed in tris-buffered saline (TBS) containing 0.5% Triton X-100 (TBS-Tx) for expression analysis, followed
- (Thermo Fisher Scientific) supplemented with 25 mM KCl and 10% fetal bovine serum from 1 to 3 days in
Methods (brief)
- collected the data. FU and MF aided in interpreting the results and worked on the manuscript. AS wrote the
Implications
This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.
Wiki pages this source may touch
- Fish — marine, predatory (tuna, swordfish, shark, king mackerel)
- Fish — marine, non-predatory (sardines, anchovies, salmon, cod)
- Mercury
- Mercury
- Cadmium
Verification notes
- Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing
wiki/sources/pages before creation. - Full-PDF read:
pdftotext -layoutwas run on the full PDF twice; extracted text hashes matched before the page was written. - Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
- Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
- HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.
Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.