Impact of Arsenic Stress on the Antioxidant System and

Overview

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Key numbers

The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:

• containing improved Zarrouk culture medium (Table S1), with a diurnal cycle of 12/12 h
• standard solution at initial concentrations ranging from 0 to 150 mg/L was added to
• centrifuge tubes. Next, 20 mL of 96% ethanol was added, and the mixture was vigorously
• The total RNA of the 70 mg/L arsenic treatment group and the control group was
• reads were generated by removing low-quality sequences reads with more than 10% of N
• The primer sequences are listed in Table S2. The SYBR Green ProTaq HS Premix qPCR Kit
• was a biphasic effect in A. platensis cells. Briefly, the growth rate of the cells under 20 mg/L
• the range of 20 mg/L to 90 mg/L As3+ , there were no significant differences in the contents
• chlorophyll was observed in the groups treated with 150 mg/L As3+ .
• The activities of SOD and CAT were significantly higher in 20 mg/L to 60 mg/L of
• As3+ concentration increased from 100 mg/L to 150 mg/L, the activities of both enzymes
• the As3+ concentration increased from 100 mg/L to 150 mg/L, the activities of both enzymes
• bosomes (Figure 5a). Meanwhile, the upregulated differentially expressed genes associated
• platensis was activated after being stressed by As3+ . Meanwhile, the results of Figure 6b
• When the As3+ concentration was 20 mg/L, the Sm value showed no significant change
• compared to the control group; when the As3+ concentration was ≥50 mg/L, the Sm value

Methods (brief)

• (30 min at −80 ◦ C; 15 min at 25 ◦ C), repeated 6 times. After the final cycle, the sample
• was centrifuged again (12,000 r/min, 4 ◦ C, 10 min), and the supernatant was collected for
• solvent immersion method (8). The algal pellets collected were the same as 2.3 in 50 mL
• with intermittent shaking every 6 h. The samples were centrifuged again (8000 r/min,
• 12,000 r/min for 15 min, and the algal paste was collected in a mortar. Then, it was ground
• Depletion Kit for Mixed Bacterial Samples (Lexogen, NH, USA), and then, all mRNAs
• used for plotting, with each sample undergoing three biological replicates. Error bars
• using the clustering method to calculate the distance between samples according
• by using the clustering method to calculate the distance between samples according to to gene
• expression (Figure 4a,b). Samples within groups were well clustered in control groups
• gene expression (Figure 4a,b). Samples within groups were well clustered in control
• samples in a given group were similar, and there was a large difference between the groups
• of samples in a given group were similar, and there was a large difference between the
• samples. In figure, the the
• right and lower sides represent sample names, the left and upper sides are sample clustering, and
• right and lower sides represent sample names, the left and upper sides are sample clustering, and the
• the squares with different colors represent the correlation between the two samples (b) Hierarchi-
• squares with different colors represent the correlation between the two samples (b) Hierarchically

Implications

This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.

Wiki pages this source may touch

• Fish — marine, non-predatory (sardines, anchovies, salmon, cod)
• Root-Vegetable Purees
• Mercury
• Arsenic

Verification notes

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Update history

The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.