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Heavy Metal Index

Differential Cell Metabolic Pathways in Gills and Liver of Fish

Source

This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus.

Page snapshot
Cited by8 pages
Metals measured5
Evidence tierB
Year2023

Overview

This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.

Key numbers

The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:

  • experiment was conducted with juvenile white seabreams, Diplodus sargus (Linnaeus, 1758), exposed
  • The white seabream, Diplodus sargus (Linnaeus, 1758), is a common demersal fish that
  • (1 H NMR) allows for the simultaneous analysis of a wide range of metabolites involved in
  • Deuterium oxide (D2 O, heavy water; 99.8 atom %D; CAS-No. 7789-20-0) for NMR
  • dimethyl-2-silapentane-5-sulfonate sodium salt (DSS; 97%, molecular weight: 218.32 g mol−1 ;
  • photoperiod. Seawater was renewed daily (around 80%), and fish were fed once a day,
  • Portugal) composed by 44% protein and 16% lipids. A solution of MeHg chloride (pre-
  • (pH 7.0, 10% D2O) with 1 mM DSS, used as internal reference, and then transferred a chemical shift
  • calibration (DSS at 0.0 ppm) of all 1 H NMR spectra acquired. The Chenomx 500 MHz library
  • and liver were presented as mean ± SD, and statistically significant differences (p < 0.05)
  • spond to mean ± standard deviation (n = 8). Significant differences (Sidak’s test; p < 0.05) in relation
  • correspond to mean ± standard deviation (n = 8). Significant differences (Sidak’s test; p < 0.05) in
  • fish gills at each experimental time (Table 1). Indeed, a significant increase in the levels of
  • (PE28). Regarding energy metabolism, a significant notable rise in lactate was noted at all
  • fish gills at each experimental time (Table 1). Indeed, a significant increase in the levels
  • (PE28). Regarding energy metabolism, a significant notable rise in lactate was noted at
  • groups (n = 6) (Dunnett’s test, * p < 0.05; ↑ and ↓ indicate an increase and decrease in respect to the
  • Valine ↑ 20% * ↑ 43% * ↓ 16%
  • Alanine ↑ 31% * ↑ 65% * ↓ 4%
  • Phenylalanine ↑ 34% * ↑ 54% * ↓ 24% *
  • Malonate ↑ 32% * ↑ 64% * ↓ 16% *
  • fish liver at each experimental time (Table 2). In regard to amino acids, a significant increase
  • groups (n = 6) (Dunnett’s test, * p < 0.05; ↑ and ↓ indicate an increase and decrease in respect to the
  • Alanine ↑ 32% * ↑ 30% * ↑ 13%
  • Creatine ↓ 20% * ↓ 39% * ↓ 27% *
  • Cystathionine ↑ 2% ↑ 79% * ↑ 112% *
  • Niacinamide ↑ 55% * ↑ 58% * ↑ 73% *
    1. Giacalone, V.M.; Pipitone, C.; Abecasis, D.; Badalamenti, F.; D’Anna, G. Movement ecology of the white seabream Diplodus sargus
  • dwelling omnivorous fishes: The case of Diplodus sargus (L.) (Osteichthyes: Sparidae). Mar. Pollut. Bull. 2018, 136, 10–21.
  • swimming behavior in fish (white seabream—Diplodus sargus). Aquat. Toxicol. 2016, 170, 400–412. (CrossRef)

Methods (brief)

  • (White Seabream Diplodus sargus) Coping with Dietary
  • experiment was conducted with juvenile white seabreams, Diplodus sargus (Linnaeus, 1758), exposed
  • Diplodus sargus) Coping with Dietary
  • The white seabream, Diplodus sargus (Linnaeus, 1758), is a common demersal fish that
  • Juvenile white seabreams (Diplodus sargus), provided by the Aquaculture Research
  • arinized Pasteur pipettes). Gills and liver were excised, and the two sets of samples stored
  • with white seabream, Diplodus
  • white seabream, Diplodus sargus,
  • samples were lyophilized,
  • total Hg (tHg) in an advanced mercury analyzer (AMA) (AMA254,
  • for total Hg (tHg) in an advanced mercury analyzer (AMA) (AMA254, LECO Instru- LECO Instruments),
  • concentrate Hgsample. ThermalThermal
  • in the sample. decomposition (around(around
  • lytically reduce Hg in the sample to its elemental form. Elemental Hg was thenontrapped
  • Hg in the sample to its elemental form. Elemental Hg was then trapped a gold
  • transported by a stream of oxygen and measured by atomic absorption
  • Hg was transported by a stream of oxygen and measured by atomic absorption spectrom- spectrometry (47).
  • partition. Afterward, samples ◦ C, resulting in

Implications

This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.

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Verification notes

  • Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing wiki/sources/ pages before creation.
  • Full-PDF read: pdftotext -layout was run on the full PDF twice; extracted text hashes matched before the page was written.
  • Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
  • Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
  • HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.

Update history

The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.

CommitDateChangeDescription
b01ec52c2026-08-04major2 sections added
d49e450f2026-08-03major5 sections added; narrative text revised