Validation of Determination by Icp-Oes Method of Mercury Residual Levels in

Overview

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Key numbers

The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:

limit (LOQ) from the validation data ranged from 0.008 to 0.043 mg kg-1. Accuracy and precision were evaluated by means
of recovery experiments at two concentration levels (30, and 50 mg kg-1), obtaining recoveries between 87.5% and 109.3%
and coefficient of variation below 10%. Also, the relative standard deviation (RSD) ranged from 0.2% to 9%. The expanded
measurement uncertainty for mercury ranged from 11.1% to 19.2%. In investigated samples, 4.8% were detected at a
did exceed the level, but 1.3% exceeded the limit recommended by the Food and Agriculture Organization/World Health
dasyon verilerinden niceleme limiti (LOQ) 0.008 ila 0.043 mg kg-1 arasında değişmiştir. Doğruluk ve kesinlik, %87.5 ile 109.3
arasında ve geri kazanım %10’un altında varyasyon katsayısı elde ederek iki konsantrasyon seviyesinde (30 ve 50 mg kg-1) geri
kazanım deneyleri yoluyla değerlendirilmiştir. Ayrıca, bağıl standart sapma (RSD) %0.2 ila %9, genişletilmiş ölçüm belirsizliği
%11.1 ile %19.2 arasında değişmiştir. Araştırılan örneklerde %4.8’i saptanabilir civa seviyesinde tespit edildi, ancak bu sevi-
metals for human (1,7). The pollution of the aquatic en- tilefish (1.45 ppm), shark (0.988 ppm), swordfish (0.976
vironment has become a worldwide problem during in ppm), and mackerel (0.730 ppm). The highest concen-
recent years (8). Aquatic is the most important of the tration observed was 4.540 ppm, seen in shark samples
need for assessing the quality of seafood. The aim of Australia). High purity (99.9%) argon was used as plasma,
It was employed 25 % SnCl2 (m/v) solution as reductant.
Samples of fish muscle from tuna species skipjack tuna (65% purity), 2-3 drops of H2O2 (30%) was added to the
thynnus) (n = 95), yellowfin tuna (thunnus albacares) lids of the teflon containers were closed and placed in
(n = 90), and albacore tuna (thunnus alalunga) (n = 100) the microwave burner. At the end of the burning pro-
All glassware was left to stand overnight in 6.5% (v/v) of determination, the limit of measurement, linear me-
nitric acid (Merck, Germany), then washed with 10% asuring range, precision, repeatability, reproducibility,
dard stock solution was prepared from Titrasol (1000 (LOQ) were calculated according to the second edition
ponding metal solution. 2% solution of HCl (Merck, Ger- Methods for Second Edition 2014.
Calibration solutions were prepared with 5 ppb, 10 ppb, Statistical Analysis
20 ppb and 40 ppb of 10 ppm Hg standard working so- The results of the mercury concentration in canned fish
Table 1. Enantiomeric separations using cyclodextrins (CDs) and derivatives as chiral selectors.
*Statistical significance: p<0.05. LOQ: Limit of Quantitation.
and sensitivity values are shown in Table 1. We can ob- reproducibility limit is calculated according to the follo-
Determination limit (LOD) and measurement limit (LOQ) covery results are given the Table 2. The recovery rate
Mercury standards (0.005ppm-0.01ppm-0.02ppm- was calculated according to the following formula:
Average recovery rate was 97 ± 2% for mercury. inty. The uncertainty from accuracy, the difference from
Linear Measuring Range cant from 1 and the uncertainty from accuracy was calcu-
In order to determine the linear measuring range, 4 dif- lated by the following 1, 2, 3 with formulas.
Table 2. Results and calculated % recovery.
0.5ppm Hg 0.3ppm Hg
Table 3. Combined and expanded uncertainty applied to analysis of certified reference material, k = 2
*Statistical significance: p<0.05. LOQ: Limit of Quantitation.
Table 4. Combined and expanded uncertainty applied to analysis of certified reference material, k = 2.
Albacore Tuna 0.079* 0.054* 0.011* <LOQ 0.22
*Statistical significance: p<0.05. LOQ: Limit of Quantitation.
u R ropriate recovery was confirmed (Table 2). The limit of
1.96 Eq (3) detection (LOD) of mercury are 0.5 and 0.3 ppm for can-
Table 3 shows results obtained for Hg in 375 samples Regular monitoring of mercury contamination in fish
Turkish Food Codex legislation from the sample of 102 the Hg level ranged from 0.015 to 0.110 mg kg-1, in

Methods (brief)

Validation of Determination by Icp-Oes Method of Mercury Residual Levels in
Türkiye’de Satışa Sunulan Konserve Balık Etlerinde Civa Kalıntı Seviyelerinin Icp-
T his study describes the residual mercury levels in canned fish marketed in Turkey. In total, 375 fish samples were ana-
lyzed by Inductive Coupled Plasma Optical Emission Spectrometry (ICP OES) for mercury residues. The quantification
limit (LOQ) from the validation data ranged from 0.008 to 0.043 mg kg-1. Accuracy and precision were evaluated by means
measurement uncertainty for mercury ranged from 11.1% to 19.2%. In investigated samples, 4.8% were detected at a
Mercury, canned fish, ICP-OES, method development.
munesi, cıva kalıntıları için Endüktif Çiftleşmiş Plazma Optik Emisyon Spektrometresi (ICP OES) ile analiz edilmiştir. Vali-
dasyon verilerinden niceleme limiti (LOQ) 0.008 ila 0.043 mg kg-1 arasında değişmiştir. Doğruluk ve kesinlik, %87.5 ile 109.3
Civa, konserve balık, ICP-OES, yöntem geliştirme.
recent years (8). Aquatic is the most important of the tration observed was 4.540 ppm, seen in shark samples
tification with ICP OES and to determine total mercury gas flows were kept at 15.0 L min-1 for plasma, 1.5 L min-
content in total 375 canned fish samples supplied from 1
paper is one part of the entire proceedings, not an in- detecting the mercury concentration by ICP-OES after
dependent document. Please do not revise any of the reducing the amount of Hg in the samples with sodi-
samples in the microwave with nitric acid. Each sample
From the homogenized sample, about 1 g was weighed
Sample collection into the teflon sample incinerator. Then, 10 ml of HNO3

Implications

This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.

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The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.

Commit	Date	Change	Description
b01ec52c	2026-08-04	major	2 sections added
d49e450f	2026-08-03	major	5 sections added; narrative text revised