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Heavy Metal Index

Transport and Toxicity of Methylmercury-Cysteine in Cultured

Source

This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus.

Page snapshot
Cited by8 pages
Metals measured5
Evidence tierB
Year2022

Overview

This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.

Key numbers

The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:

  • iations. proximately 80% of anthropogenic Hg is released into the air and subsequently deposits
  • carried out at 37 ◦ C for various times between 5 and 60 min. Results are presented as mean ± SE.
  • 5 µM (14 C)-MeHg-Cys in the presence of unlabeled MeHg-Cys. Results are presented as mean ± SE.
  • dependent (B) conditions. Results are presented as mean ± SE. Data represent 3 experiments
  • as mean ± SE. Data represent 3 experiments performed in triplicate. *, significantly different (p <
  • control. Results are presented as mean ± SE. Data represent 3 experiments performed
  • control. Results are presented as mean ± SE. Data represent 3 experiments performed in triplicate. in tripli-
  • confirmed the presence of oxidative stress (Table 1). The GSH:GSSG ratio decreased as the
  • NF-κB (B), SOD1 (C), Caspase 8 (D), and HIF-1 (E). Results are presented as mean ± SE. Data
  • NF-κB (B), SOD1 (C), Caspase 8 (D), and HIF-1 (E). Results are presented as mean ± SE. Data repre- repre-
  • by approximately 70% while the sodium-dependent uptake of MeHg-Cys was inhibited
  • by 80%. This finding suggests that only about 10% of MeHg-Cys uptake is mediated
  • dissociation in 0.25% saline (Life containing
  • cells were washed twice with DMPS and were subsequently solubilized with 1% sodium
  • 16 h at 37 ◦ C in a humidified atmosphere of 5% CO2 . Subsequently, the cells were washed
  • humidified atmosphere containing 5% CO2 . Following this incubation, solubilization buffer
  • (10% Triton X-100, 0.1 N HCl in isopropyl alcohol) was added to each well, and the mixture
  • a 10% Tris-HCl gel (Bio-Rad, Hercules, CA, USA). The protein was then transferred to a
  • replicates, and each experiment was performed a minimum of three times (n = 9). Four

Methods (brief)

  • scraping, and lysed in a sonicator for 10 min. The samples were centrifuged for 10 min
  • at 8000× g at 4 ◦ C, and the supernatant was collected. The protein concentrations were
    1. Safiulina, D.; Veksler, V.; Zharkovsky, A.; Kaasik, A. Loss of mitochondrial membrane potential is associated with increase in

Implications

This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.

Wiki pages this source may touch

Verification notes

  • Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing wiki/sources/ pages before creation.
  • Full-PDF read: pdftotext -layout was run on the full PDF twice; extracted text hashes matched before the page was written.
  • Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
  • Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
  • HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.

Update history

The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.

CommitDateChangeDescription
b01ec52c2026-08-04major2 sections added
d49e450f2026-08-03major5 sections added; narrative text revised