Dietary Selenomethionine Reduce Mercury Tissue Levels

Overview

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Key numbers

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in the intestines, distributed to all organs and excreted via feces (3,4). A range of toxic
supplementary SeMet (2.5 mg Se kg−1 , see Table 1 for experimental design). Main effects
Table 1. Experimental design and dosages of MeHg and SeMet.
No MeHg Ctr (n = 6) Se (n = 6)
Low dose (LD) 1 MeHg LD (n = 6) Se + MeHg LD (n = 6)
High dose (HD) 2 MeHg HD (n = 6) Se + MeHg HD (n = 6)
physiological parameters were assessed during the trial (Figures S1 and S2 and Table S1). No
in the Se + MeHg HD group compared to the MeHg HD group were reduced by 22%, 23%
and 24% in tibialis, cortex and quadriceps femoris, respectively. Additionally, reduced level
presented as means with 95% CI (n = 6). The box in each graph denotes p-values from two-way
The results are presented as means with 95% CI (n = 6). These data did not pass the assumption of
(n = 4/group) and RNA sequencing (n = 5/group) analyses of brain tissue of adolescent
(QOE); analysis outputs are provided in Tables S2 and S3. Two-way ANOVA (Table 2)
Table 2. Summary of the numbers of differentially expressed proteins and RNA transcripts (p ≤ 0.05)
(IPA), upon which findings are described in Table 3.
Table 3. Summary of diseases/functions, canonical pathways and upstream regulators from Ingenuity Pathway Analysis (IPA) based on the proteins (149 proteins)
disease further described in Table 4. ** p-value < 0.01, *** p-value < 0.001.
Table 4. Overview of selected features related to the overall categories of cell plasticity, oxidative
corresponding p-values and direction of regulation are displayed in Table 4.
groups (n = 6 per group) based on body weight and fed experimental diets three times
in (41) and Table 1 provides a summary of experimental design and supplemented levels of
feeds were determined to be 0.088 mg kg−1 (n = 9), mercury levels in the control feeds were
below the limit of quantification (LOQ) for the instrument (n = 4). The LD-, HD-MeHg and
MA, USA) as described by Julshamn et al. (100). The LOQ of this method is 0.005 mg kg−1
been described (41). All RNA transcripts can be found in Table S3. Raw RNAseq reads
Dorne, J.; Grohmann, L.; Herman, L.J.E.S.P. EFSA Scientific Colloquium 24–’omics in risk assessment: State of the art and next

Methods (brief)

the brain, were sampled for mercury analyses. Hippocampi were collected and analyzed using
To assess mercury excretion, feces from all mice were collected during the last week
Inductively coupled plasma mass-spectrometry (ICP-MS) was used as described else-
below the limit of quantification (LOQ) for the instrument (n = 4). The LD-, HD-MeHg and
collected. Liver, kidneys, hippocampus, cortex, cerebellum, tibialis and quadriceps femoris
were sampled, weighed, snap-frozen in liquid nitrogen and stored at −80 ◦ C until further
processing. Feces from the 10th week of the experiment were collected from the bedding
mercury analysis (DMA-80, Milestone, Sorisole, Italy) as described elsewhere (41,101). THg
plasma mass spectrometry (ICP-MS; Thermo iCAP Q, ThermoFisher Scientific, Waltham,
MA, USA) as described by Julshamn et al. (100). The LOQ of this method is 0.005 mg kg−1
toxicity (102,103). A total of 24 hippocampus samples (four mice per exposure group) were
prepared for proteomic analysis. Sample preparation and protein mass spectrometry were
Five hippocampus samples from each group were included for RNA-sequencing
(RNA-seq), harvested from the same mice as the proteomics samples. Procedures, kits,
and Se groups were not spiked with MeHg and accordingly showed levels below LOQ
Dorne, J.; Grohmann, L.; Herman, L.J.E.S.P. EFSA Scientific Colloquium 24–’omics in risk assessment: State of the art and next
Aaseth, J. Insights into the Potential Role of Mercury in Alzheimer’s Disease. J. Mol. Neurosci. 2019, 67, 511–533. (CrossRef) (PubMed)
1. Kryscio, R.J.; Abner, E.L.; Caban-Holt, A.; Lovell, M.; Goodman, P.; Darke, A.K.; Yee, M.; Crowley, J.; Schmitt, F.A. Association of

Implications

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Update history

The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.

Commit	Date	Change	Description
b01ec52c	2026-08-04	major	2 sections added
d49e450f	2026-08-03	major	5 sections added; narrative text revised