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Heavy Metal Index

Arsenic Activates the ER Stress-Associated Unfolded Protein

Source

This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus.

Page snapshot
Cited by3 pages
Metals measured1
Evidence tierB
Year2022

Overview

This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.

Key numbers

The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:

  • drinking water is 10 ppb (<0.1 µM, 10 µg/L). However, there are areas in the US, India,
  • Bangladesh, China, Chile, Mongolia, Mexico, and Argentina where more than 50 ppb of
  • cancer (NSCLC) forms about 80% of the total lung cancer cases, which can be further
  • Meanwhile, a metabolic change was observed in the As3+ -induced CSCs compared
  • of the CSCs (10–12). Meanwhile, induction of the CSCs by As3+ is made by a relatively
  • 5% Fetal Bovine Serum, 1% L-Glutamine and 1% Penicillin/Streptomycin in a humidified
  • incubator at 37◦ C and 5% CO2 . The cells were starved with serum-free medium (overnight),
  • primer, 50 -CATGAGGTCCACCTGTTGCTGTAGCC-30 . PCR products were run on 1% agarose
  • DMEM media. The plate was coated with 0.1% gelatin prior to cell seeding to improve cell
  • After the cells reached 70–80% confluency, the media was changed to serum-free DMEM
  • dogenous peroxidase activity, slides were incubated with 1.5 to 3% H2 O2 in PBS for 20 min
  • binding of immunoglobulin, slides were incubated with a solution containing 5% goat
  • serum, 0.2% Triton X-100 in PBS for 2 h at room temperature, followed by incubation with
  • ware. One-way Anova with 95% confidence interval followed by Tukey’s post hoc test
  • as mean values with SEM. A p-value of < 0.05 was considered statistically significant.
  • arrows). For the UBE2G2 protein, although the induction is marginal, there is a notable
  • notable decrease in both ATF6 and ATF6B in the Nrf2 KO cells relative to the WT cells
  • have set an arsenic limit of 10 ppb in drinking water. Despite the regulations, arsenic

Methods (brief)

  • gels with DNA ladders. Samples without cDNA or RT templates served as negative controls.
  • The protein samples were prepared using 4 × LDS sample buffer (Invitrogen, Carlsbad, CA,
  • at 95 ◦ C for 5 min before separation by SDS-PAGE gel. Separated samples were then
  • We observed that of the 48 patients’ samples (cancer and matched non-cancer tissue pair),
  • Q.Z., B.A., W.Z., Y.Q. and C.T. collected and analyzed data; M.H. provided instruction and technical

Implications

This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.

Wiki pages this source may touch

Verification notes

  • Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing wiki/sources/ pages before creation.
  • Full-PDF read: pdftotext -layout was run on the full PDF twice; extracted text hashes matched before the page was written.
  • Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
  • Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
  • HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.

Update history

The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.

CommitDateChangeDescription
b01ec52c2026-08-04major2 sections added
d49e450f2026-08-03major5 sections added; narrative text revised