Developmental Exposure to Low Concentrations of

Overview

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Key numbers

The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:

• range of behavioural effects including locomotor activity and stress-response; (3) they
• terminal anaesthesia with tricaine (300 mg/L) followed by decapitation.
• H2 O was purchased from Alfa Aesar at a concentration of 1000 ppm (4 mM) and volume of
• 5 mL. A working dilution of 100 ppm (400 µM) was prepared in a glass vial and kept in 4 ◦ C.
• treatment consisted of three dishes (replicates). Table 1 lists all exposure experiments
• 100% mortality within 24 h, and exposure to 300 nM caused 100% mortality by 4 dpf (data
• Table 1. List of MeHg exposure experiments.
• 0 nM >95% • All euthanised at 6 dpf
• 30 nM 50 embryos × 3 dishes >95% Due to technical difficulties- behaviour
• 5 nM >95% 30 nM- Due to low survival at juvenile
• 10 nM = 300 per treatment >95% adult adult testing
• 5-Larval RNA 10 nM >95% Non
• the treatments detailed in Table 1. Exposure solution was replaced every 24 h until 4 dpf,
• in diameter, made of Plexiglas and arranged in a 2 × 2 setup. White opaque partitions
• 5 (see Table Larvalof 10
• 10 s (45 cycles). Table 2 lists the primers used to amplify the following transcripts: β-actin,
• Table 2. List of primers used in Real-Time RT qPCR.

Methods (brief)

• DA neurons in our samples (37).
• Fertilized eggs, obtained by group breeding using in-tank inserts were collected,
• 0 nM >95% • 5 larvae from each dish sampled
• were sampled for testing out of the treatment dishes and euthanized immediately after
• 10 samples before and after every sample point in order to exclude slight movements that
• Two types of samples were collected for RNA extraction, whole larvae shortly after
• exposure, samples werefrom
• Larval samplesfromwereadult fish at
• sampled at from4 dpf,
• and snap sampled from each
• Samples were Fisherin −80
• − Ct(β-actin) )treated sample − (Ct(GOI) − Ct(β-actin) )control average (GOI, gene of interest;
• sample was calculated against the non-exposed control average.
• 29. Aaseth, J.; Wallace, D.R.; Vejrup, K.; Alexander, J. Methylmercury and developmental neurotoxicity: A global concern. Curr. Opin.

Implications

This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.

Wiki pages this source may touch

• Fish — marine, non-predatory (sardines, anchovies, salmon, cod)
• Shellfish (shrimp, crab, lobster, clams, oysters, mussels)
• Mercury
• Mercury

Verification notes

• Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing wiki/sources/ pages before creation.
• Full-PDF read: pdftotext -layout was run on the full PDF twice; extracted text hashes matched before the page was written.
• Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
• Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
• HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.

Update history

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