Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- A monolayer of cells with a confluence of about 100% was obtained by cultivating the HepG2 & Wrl-68 treated
- underwent further culture at 37 °C with 5% CO2 in an incubator. An inverted microscope (ECLIPSE Ti2, Nikon)
- membrane were counted, photographed, and stained with methanol and 0.1% crystal violet.
- amount protein (30 μg) was resolved on 10% SDS gel and was transferred to nitrocellulose membrane. One
- was incubated for 1 h in suitable HRP conjugated secondary antibodies. Clarity western ECL substrate (cat:
- MgCl2, and 6% glycerol was used to attach protein to DNA. The reaction mixture was incubated at 4 °C for
- 30 min. The DNA–protein complex was resolved on a 1% agarose gel at 3 Volt/cm for approximately 1–2 h at
- Graph Pad Prism 5 software was used to plot and analyze graphs (Graph Pad Software, Inc.). The mean ± SEM
- Table.1:. Energy obtained during docking analysis of ATO as ligand molecules and hTERT as target protein.
- concentrations ranges from (0-10micromolar). Obvious dose-and time-dependent inhibition of growth was
- site or make it unfit for its ligands (Figs. 1, 2 and Table 1). Moreover, MD simulation analysis indicates more
- of ATO to hTERT and it stable with one H-bond at end of MD simulation as shown in Fig. 3. According to
- Statistical significance 3b. was determined using a two-tailed unpaired t-test (n = 3) or one-way ANOVA
- biological processes40. Deregulation of apoptosis can result in a wide range of clinical disorders, including
- the ends of chromosomes that progressively shorten with each cell division48. By maintaining stable telomeres,
- been demonstrated to be hyperactive in 85–90% of cancers, including HCC, it has gained recognition as a tumor
- using the following formula: wound healing rate (%) = (1 − (wound area at 48-h time point/wound area at
- the 0-time point)) × 100. Statistical significance was determined using a two-tailed unpaired t-test (n = 3) or
- was determined using a two-tailed unpaired t-test (c, n = 3), (** p < 0.01, *** p < 0.001). Data are mean ± SEM.
- significance was determined using a two-tailed unpaired t-test (C, n = 3) (** p < 0.01, *** p < 0.001). Data are
- Statistical significance was determined using a two-tailed unpaired t-test (7a, n = 3) or one-way ANOVA
- followed by Tukey’s post-test (7b, n = 5) (** p < 0.01, *** p < 0.001). Data are mean ± SEM. The original blots/gels
- was determined using a two-tailed unpaired t-test (8b, n = 3). The original blots/gels are presented in
Methods (brief)
- Total protein was isolated from cell samples using RIPA buffer (Cell Signalling Technology, Inc.). The
- Reverse Transcriptase with DNAas shown in supplementary Fig. S.1. The target enzyme (hTERT), effectively
- terial and collected the data. Formal analysis and investigation were carried out by Archana Chaudhary, and
Implications
This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.
Wiki pages this source may touch
Verification notes
- Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing
wiki/sources/pages before creation. - Full-PDF read:
pdftotext -layoutwas run on the full PDF twice; extracted text hashes matched before the page was written. - Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
- Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
- HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.
Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.