Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- rafalima@ufpa.br dose of 0.375 mg/kg/day, for 45 days. They were then submitted to motor evaluation
- Male Wistar rats (n = 40; 90 days old) were obtained from
- water or mercury chloride (HgCl2 ) (dose of 0.375 mg/kg/day)
- samples and the values obtained ranged from a confidence in a fluorescence microplate reader (Victor 2, Perkin Elmer)
- interval of ±10% (r: 0.9992). every 5 min for 1 h. Background fluorescence was determined
- After this period, the specimens were washed with 50% alcohol,
- (0.1% naphthyl-ethylene diamine and 1% sulfanilamide in 5% immunohistochemical analysis. Immunohistochemistry
- Lipid peroxidation was estimated as the levels of followed by endogenous peroxidase blocking in 3% hydrogen
- detailed earlier by Esterbauer and Cheeseman (1990). Briefly, then washed in a 0.05% Tween PBS solution for 5 min, three
- an aliquot of crude homogenate was centrifuged at 2500 g for times and incubated with 10% normal horse serum in PBS
- percentages of the mean of the control group. After the reaction, the sections were washed twice in PB 0.1M,
- All data (n = 20 animals per group for behavioral tests and n = 10
- After the motor test, part of the animals (n = 10 animals per body weight was performed using one-way analysis of variance
- saline 0.9% solution followed by 4% paraformaldehyde in 0.2 for the oxidative balance data in the group exposed to HgCl2 .
- p = 0.0012) (Figure 6A). A negative correlation was also mean ± standard error of (A) latency (s) and (B) failure numbers. ∗ p < 0.05
- Results are expressed as mean ± standard error. ∗ p < 0.05 compared to the
- Wistar rats (g). Results are expressed as mean ± standard error after the (Figure 8) and astrocytes (GFAP) (Figure 9) indicated that
- and average body weight of newborns no less than 85% of the challenge imposed on the animal in crossing narrow beams
- Results are expressed as mean ± standard error of the number of cells exposed animals (Figures 5, 6). Moreover, this is the first
Methods (brief)
- (Szász et al., 2002; Huang et al., 2011; Chehimi et al., 2012), due to For mercury measurement, each homogenized sample of motor
- the immaturity of the BBB. In contrast, studies in adult organisms cortex was weighted (0.5 g maximum of wet weight) in a sample
- are scarce (Mello-Carpes et al., 2013; Teixeira et al., 2014a). digestion bottle, and 1 mL of distilled water, 2 mL of nitric
- the behavioral, tissue and biochemical level to assess the possible solution in two sample digestion bottles and (corresponding to 0
- FIGURE 1 | Sample description and experimental stages. Description of the sample and model of exposure to HgCl2 ; Beam walking motor test; division of
- the sample test solution was followed. Total mercury content analyzed through ROS determination in samples incubated or
- in the samples was estimated by wet digestion, reduction and not with a peroxyl radical generator. The detailed methodology is
- cold vapor atomic absorption spectrometry (Semi-automated described in Lowry et al. (1951) and Amado et al. (2009). Peroxyl
- Mercury Analyzer, model Hg-201, Sanso Seisakusho Co. radicals were produced by thermal (35◦ C) decomposition
- and Nishimura, 1991): total mercury concentration in the sample (H2DCF-DA) at a final concentration of 40 mM. H2DCF-DA
- (µg/g) = 0.10 µg × (test sample – blank sample)/(standard passively diffuses through cellular membranes and once inside
- sample – blank sample) × dilution factor × 1/sample weight the acetates are cleaved by intracellular esterases. Thereafter,
- samples and the values obtained ranged from a confidence in a fluorescence microplate reader (Victor 2, Perkin Elmer)
- Sample Preparation along the time of the measurement (x axis), after adjusting
- The motor cortex samples collected for biochemical analysis FU data to a second order polynomial function. The results
- then thawed and resuspended in Tris-HCl 20 mM, pH 7.4 at sample with and without ABAP addition and standardized to
- samples in exactly the same way. The lysate was stored at −80◦ C addition of ABAP, indicate high competence in neutralizing
- levels as described in Green et al. (1982). Briefly, the samples were Immunohistochemistry
Implications
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Verification notes
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Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.