Role of Sulfate Transporters in Chromium Tolerance in

Overview

This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.

Key numbers

The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:

• SaSULPs and 64.8% similarity. Analysis of the amino acid sequence
• 77.3% SaSULP2
• SaSULP1SaSULP1, (25.5% identifying
• 2.2.2.The SaSULP2 gene was homologous to C. reinhardtii SulP2, with a 67.1% identity
• analysis throughshowed 55.9%
• per site. This analysis involved 117 amino acid sequences (Table S1) for a total of 1769 positions in
• per site. This analysis involved 117 amino acid sequences (Table S1) for a total of 1769 positions in the
• per site. This analysis involved 103 amino acid sequences (Table S1) for a total of 1036 positions in
• finalThis analysis involved 103 amino acid sequences (Table S1) for a total of 1036 positions in the
• (Table S1) for aa total
• significant decrease after 24 h of metal exposure, but remained substantially stable low but
• after 24 h of metal exposure, but remained substantially stable within
• A comparison between SaSULTR1 and SaSULTR2 copy number (Table 1) indicated
• inducing SaSULTR2 and leaving unchanged SaSULTR1 transcription (Table 1).
• Table 1. Copy number/ng cDNA oligo(dT)20 encoding SaSULTR2 and SaSULTR1 transporter in
• respectively) (Table 1). Despite the greater SaSULTR2 relative increase observed in the Cr t
• strain (Table 1). After 24 h from nutrient re-supply the total copy number of SULTRs was
• SaSULTR1 expression, however, prevailing in the Cr-t and SaSULTR2 in the wt (Table 1).
• which SLT2 and SLT3 are arranged in tandem in a head-to-tail orientation with a partial
• The GenBank accessions of sequences used for the analysis are reported in Table S1. The
• the wt population with a sublethal concentration (1 mg/L) of hexavalent chromium (Cr(VI))
• EDTA; 2% β-Mercaptoethanol) was added. Following an incubation for 40 min at 58 ◦ C,
• Table 2. List of primers used for aqPCR of studied genes encoding sulfur transporters.
• quantification (Table 2). The single amplified PCR product was verified based on size in
• a 3% agarose gel under UV illumination. The gel band containing the DNA target was

Methods (brief)

• included most of the wt samples, whereas the second one grouped Cr-t samples and wt
• into two subgroups: the controls and the Cr-exposed samples, regardless of the treatment
• the controls at shorter growth time cluster together with the samples exposed to Cr(VI)
• included the control at 48 h, all the samples exposed to 2 mg Cr(VI)/L and the wt exposed
• group included the controls and the 1 mg Cr(VI)/L exposed wt samples. Very intriguingly,
• the second one grouped all the Cr-t samples and all the 17wtof 28samples exposed to 2 mg
• Cr(VI)-treated samples generated a sub-cluster separate from controls, regardless of the
• samples clustered together with the Cr-t samples. Probably this happened because in
• (called T0) in +S and in −S medium, cells of both strains were washed and collected by
• strains, were collected by centrifugation, twice washed with double distilled water, frozen
• the tubes were centrifugated for 10 min (3000× g). After, the supernatant was collected in
• 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Only RNA samples with
• determined by reading the standard series with the Ct values of each sample and reported
• 58. Haas, F.H.; Heeg, C.; Queiroz, R.; Bauer, A.; Wirtz, M.; Hell, R. Mitochondrial serine acetyltransferase functions as a pacemaker of

Implications

This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.

Wiki pages this source may touch

• Fish — marine, predatory (tuna, swordfish, shark, king mackerel)
• Fish — marine, non-predatory (sardines, anchovies, salmon, cod)
• Shellfish (shrimp, crab, lobster, clams, oysters, mussels)
• Seaweed/kelp foods (nori, wakame, kombu, dulse — as food products)
• Cadmium
• Chromium

Verification notes

• Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing wiki/sources/ pages before creation.
• Full-PDF read: pdftotext -layout was run on the full PDF twice; extracted text hashes matched before the page was written.
• Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
• Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
• HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.

Update history

The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.