Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- expPABPN1 were identified. These differences are asso- medium (Invitrogen), supplemented with 20% fetal bo-
- the reduced amount of the soluble form is likely to be in a humidified 10% CO2 air atmosphere. Terminal differ-
- becco’s modified Eagle’s medium containing 5% horse
- Total RNA was extracted from myotube cultures after (pH 6.8), 10 mmol/L dithiothreitol (freshly added), 2%
- cated in Table 1. The specificity of RT-PCR products was de-ubiquitinating enzyme (DUB) inhibition, 10 mmol/L
- Table 1. Primers Used for RT-qPCR Analysis
- turated and resolved by SDS-PAGE on 10% polyacryl- Myotube Cultures at Low Expression Levels
- Immunofluorescence and Fluorescence in Situ the use of the 3= half of the HBB gene.43– 46 Stable clones
- fusion, intense fluorescent foci were detected (INIs, Fig- represented ⬎10% of the genes in a given pathway (see
- ure 1F and Figure 2A) and were visualized by either Supplemental Table S1 at http://ajp.amjpathol.org). In a
- PABPN1. In addition, PABPN1 foci colocalized with ubiq- OPMD-deregulated pathways (see Supplemental Table
- of differences in PABPN1 transgene expression levels (see ubiquitin proteasome system (Table 2). More important,
- Supplemental Table S2 at http://ajp.amjpathol.org). These these pathways are commonly deregulated in patients
- WTA-deregulated genes were mapped to 41 KEGG path- with D7E and OPMD (see Supplemental Table S1 at
- model of OPMD (see Supplemental Table S2 at http://ajp. are involved in OPMD disease etiology.
- ulated because of PABPN1 expression levels (Table 2). pathway in a cross-species study29 for OPMD. In the D7E
- paring the effect of expression level (Figure 3). In the expPABPN1 expression (see Supplemental Table S1 at
- A17.1 mouse, apoptosis was listed among the top 20 de- http://ajp.amjpathol.org). Approximately 20% of the pro-
- has been subjected to extensive studies.22,47,48 In our D7E among which 80% were down-regulated. Validation of
- plemental Table S1 at http://ajp.amjpathol.org). The acti- is regulated by the proteasome. In this experiment,
- reported in affected muscles of patients with OPMD, we imate 20% increase in nuclei containing INIs in D7E
Methods (brief)
- Kirsten R. Straasheijm,* Rinse Klooster,* ton’s disease,1 polyalanine tract expansions have also
Implications
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Verification notes
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Update history
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