Overview
This in vitro study compared the cytotoxicity of commercially pure titanium (cpTi), silver-palladium (Ag-Pd), and nickel-chromium (Ni-Cr) dental alloys on human gingival fibroblasts (HGF) using the MTT assay in both DMEM culture media and artificial saliva. Ag-Pd showed intermediate cytotoxicity between the biocompatible cpTi and the more cytotoxic Ni-Cr alloy.
Key numbers
- Ag-Pd HGF viability in DMEM: 77.6% (range 75.8-79.4%)
- Ag-Pd HGF viability in artificial saliva: 65.3% (range 63.5-66.5%)
- Ag-Pd MTT absorbance: DMEM 0.316 +/- 0.006; artificial saliva 0.294 +/- 0.002
- cpTi HGF viability: DMEM 91.8%; artificial saliva 90.2% (least cytotoxic)
- Ni-Cr HGF viability: DMEM 67.5%; artificial saliva 54.1% (most cytotoxic)
- Statistical significance: p less than or equal to 0.05 for Ag-Pd vs control and cpTi vs Ag-Pd
Methods (brief)
Human gingival fibroblast (HGF) cell culture. Alloy discs (cpTi, Ag-Pd, Ni-Cr) incubated with HGF cells in DMEM and artificial saliva media for defined periods. MTT assay at endpoint. Optical density measured at 570 nm. Statistical comparison by ANOVA with post-hoc testing.
Implications
This page makes the source discoverable for palladium evidence routing. Demonstrates that Ag-Pd dental alloy causes moderate cytotoxicity to human gingival fibroblasts in vitro, with greater cytotoxicity in artificial saliva than DMEM, relevant for understanding local tissue responses to Pd-containing dental restorations in the oral environment.
Related evidence
Update history
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