Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- FBS used in this study (Sigma, SA, USA. Lot.BCCC5944) containing approximately 8 ppb of
- Medium (4.5 g/L Glucose) with L-Gln and Sodium Pyruvate with 10% fetal bovine serum, 100 U/mL
- and 100 μg/mL penicillin–streptomycin in a humidified incubator at 37℃, 5% CO2, and 95% ambient
- 10 % or 20 % and cultured to an 80 % sub confluent state. In the present study, HepG2 was seeded
- then replaced with media containing 10% alamarBlue reagent (Thermo Fisher, OR, USA) and
- blocked with 5% skim milk. The membrane was rinsed with TBST and, protein immunoblotting was
- and 1.8% acetonitrile was used to separate GSH. The flow rate was fixed at 0.25 mL/min, and the
- of concentrated nitric acid (70%) and decomposed using a microwave digestion system (ETHOS
- volume of 1.0 mL (final nitric acid concentration: 17.5 %) and subjected to ICP-MS analysis. Arsenic
- 17.5% nitric acid and continuously introduced online with the samples. Arsenic concentrations were
- probability value was 5% or less. The data are expressed as mean±standard deviation (S.D.). Three
- to As(III), whereas curcumin had no notable cytoprotective effect under similar conditions. GO-Y015
- Y022 has weak potential to activate Nrf2, which indicate that methoxy gropus which contributable
- assay was performed. n=3, Mean±S.D., **P < 0.01, ****P < 0.0001 vs. DMSO, One-way ANOVA with
- Curcumin and GO-Y015 were added to achieve 0–8 µM. After 6 h, alamarBlue assay was performed. n=3, Mean
- containing As(III) at 0–120 µM. After 16 h, alamarBlue assay was performed. n=3, Mean±S.D., **P < 0.01,
- µM. After 16 h, alamarBlue assay was performed. n=3, Mean±S.D., **P < 0.01, ****P < 0.0001 vs. DMSO,
- µM) sulforaphane (5 µM) were added. After 6 h, cells were harvested using 2% SDS buffer for protein and analyzed
- by Western blot (n = 3). Data were shown as mean±S.D., *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs.
- Then the cells were harvested using 2% SDS buffer for protein and analyzed by Western blot (n = 3). mean±S.D.,
- using 2% SDS buffer for protein and analyzed by Western blot (n = 3). (h) HepG2 cells were pre-treated with GO-
- High Glucose DMEM containing As(III) at 0–150 µM. After 16 h, alamarBlue assay was performed. n=3, Mean±
- cells were harvested using ISOGEN II (for RNA) and analyzed by RT-qPCR (n = 3). Data were shown as mean±
- alamarBlue assay was performed. n=3, Mean±S.D., **P < 0.01, ****P < 0.0001 vs. DMSO, One-way ANOVA
- at 0–150 µM. After 16 h, alamarBlue assay was performed. n=3, Mean±S.D., **P < 0.01, ****P < 0.0001 vs.
- concentration was used for the normalization (n=3). Data were shown as mean±S.D.*P < 0.05, **P < 0.01, ***P
- normalization (n=3). Data were shown as mean±S.D.*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
Methods (brief)
- on the Nrf2–GSH–MRP axis. Furthermore, ICP-MS analysis demonstrated that GO-Y015
- (Nacalai Tesque, Kyoto, Japan) as the standard. Protein samples were separated by sodium dodecyl
- water (Qiagen, Hilden, Germany). The cDNA samples were diluted 1:10 and used as templates. For
- coupled with an electrochemical detector (HPLC-ECD)
- chromatography (HPLC)-electrochemical detector (ECD) analysis, which was slightly modified from
- cells. After centrifugation, the supernatant was subjected to an HTEC-500 HPLC-ECD system
- Chemical, Osaka, Japan). The sample was measured at a dilution ratio that was linear within the
- 2.7 Determination of Arsenic concentration by ICP-MS
- Samples were acid-digested prior to elemental analysis. Briefly, each sample was mixed with 250 μL
- of concentrated nitric acid (70%) and decomposed using a microwave digestion system (ETHOS
- EASY, Milestone General, Kanagawa, Japan). Digestion was carried out in sealed high-pressure
- quartz vessels at 160 °C with a maximum pressure of 80 bar for 30 min. Following digestion and
- volume of 1.0 mL (final nitric acid concentration: 17.5 %) and subjected to ICP-MS analysis. Arsenic
- 17.5% nitric acid and continuously introduced online with the samples. Arsenic concentrations were
- quantified by ImageJ 1.53i (National Institutes of Health, USA). Detailed sample sizes and statistical
- high-performance liquid chromatography coupled with an electrochemical detector (HPLC-ECD),
- accumulation. We measured the total As level in cells using ICP-MS. First, we pretreated HepG2
- Y015 (5 µM) sulforaphane (5 µM), or DMSO were added at 0 h. Samples were collected after 3 and 6 h of incubation.
Implications
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Verification notes
- Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing
wiki/sources/pages before creation. - Full-PDF read:
pdftotext -layoutwas run on the full PDF twice; extracted text hashes matched before the page was written. - Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
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Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.