Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- BSF-4 achieved 89.15% reduction of 20 mg/L Cr(VI) within 72 h, with Cr(III) identified as
- Attribution (CC BY) license Environmental Quality Standard (GB15618-2018)” (9) is set at 350 mg/kg. The permissible
- concentration of Cr(VI) in surface water is 0.05 mg/L. The U.S. Environmental Protection
- 0.1 mg/L for Cr(VI) in public water systems under the National Primary Drinking Water
- For example, in the presence of 5 mg/L Cr(VI), suspension cells of Arthrobacter sp. Sphe3
- can achieve a Cr(VI) reduction rate of 94% (22). Bacillus cereus was able to reduce all Cr(VI)
- with an initial mass concentration of 10~50 mg/L, which laid a foundation for the research
- on chromium reduction (23). Aspergillus niger can remove 70% of 500 mg/kg of chromium
- land, and the Cr(VI) tolerance mass concentrations were up to 1000 mg/L (25,26). It
- concentration of 20 mg/L, the reduction efficiency of Cr(VI) is as high as 89.15% within
- NaCl, pH 7.0) standard Luria–Bertani medium. LB medium with 1%, 3%, 5%, 7%, 9%,
- 11% and 13% gradient salt concentration was used sequentially to screen the salt-resistant
- strains, then the 13% NaCl concentration was cultured by dilution coated plate method,
- LB agar plates 3 times), and then added with 50% glycerol (v/v, 1:1 ratio with bacterial
- NaCl of 1–13% (w/v) was added to the medium, respectively, inoculated with 1%
- at a 1% inoculum ratio and incubated at 35 ◦ C with shaking at 150 rpm to assess strain
- concentrations (0, 20, 40, 60, 80, 100, and 200 mg/L) for Cr(VI) resistance test.
- centration of 0, 20, 60, and 100 mg/L, and cultured in an oscillating incubator at 35 ◦ C
- spike recovery tests (85–115%) to ensure measurement accuracy.
- The cell morphology under different Cr(VI) stresses (0, 20, 100 mg/L) was observed
- and 20 and 100 mg/L Cr(VI), respectively, cultured at 35 ◦ C and 150 rpm to the logarithmic
- phosphate buffer for 2~3 times. Add 2.5% glutaraldehyde solution and mixed homoge-
- nutrient medium containing 20 mg/L Cr(VI) and cultured at 30 ◦ C and 150 rpm for 48 h.
- The rest cell suspension was added with 20 mg/L Cr(VI), and the other components
- of the suspension were inoculated into the nutrient medium containing 20 mg/L Cr(VI),
- 20 mg/L Cr(VI) in logarithmic stage, and the bacterial samples at 12 h, 24 h, 36 h and 48 h
- were subjected to strict quality control by means of Agilent 2100 bioanalyzer (Santa Clara,
- When the salt concentration was as high as 13%, the bacterial growth and reproduction
- and can survive within the salt concentration range of 1% to 11%. At the same time, its
- most suitable salt concentration for growth is 3%. It was cultured under optimal salt
- tolerate at least 200 mg/L of Cr(VI), but with the increase in Cr(VI) concentration, cell
- of Cr(VI) shows that when the initial concentration of BSF-4 is 20, 60, and 100 mg/L,
- the reduction efficiency of Cr(VI) within 72 h is 89.15%, 66.95%, and 44.42%, respectively
- 20 mg/L Cr(VI) (Figure 2b), and the cells were significantly elongated after 100 mg/L Cr(VI)
- of 20 mg/L Cr(VI) (Figure 2b), and the cells were significantly elongated after 100 mg/L
- tion for 24 h with different Cr(VI) concentrations. (a) 0 mg/L Cr(VI); (b) 20 mg/L Cr(VI);
- 24 h with different Cr(VI) concentrations. (a) 0 mg/L Cr(VI); (b) 20 mg/L Cr(VI); and (c) 100 mg/Land
- (c) 100 mg/L Cr(VI).
- of BSF-4 under Cr(VI) stress. (a) TEM of 20 mg/L Cr(VI); (b) Mapping of 20 mg/L Cr(VI); (c) EDS
- of 20 mg/L Cr(VI); (d) TEM of 100 mg/L Cr(VI); (e) Mapping of 100 mg/L Cr(VI); and (f) EDS of
- 100 mg/L Cr(VI).
- concentration of 20 mg/L, the extracellular residual Cr(VI) concentration was 7.933 mg/L,
Methods (brief)
- Saline soil samples were collected from Binzhou, Shandong Province, China
- (37.22◦ N, 118.02◦ E). Take 5 g of soil sample into a 250 mL sterilized conical flask, add
- growth. Samples were collected at 4 h intervals for growth monitoring, and the assay was
- chromium content was determined through a hot-block digestion system in accordance
- distance was moderately adjusted for optimized imaging across samples).
- metabolites were sampled after 0.22 µM.
- part was resuspended with deionized water, that is, to obtain an intact cell sample.
- was taken as the crude enzyme extract sample.
- 20 mg/L Cr(VI) in logarithmic stage, and the bacterial samples at 12 h, 24 h, 36 h and 48 h
- experiment groups, and one control group W0 which without Cr(VI), a total of 15 samples
- transcriptome sequencing (sequencing results are provided in Text S1). The RNA samples
- that two distinct peaks were obtained from the extracellular polymer sample of strain
- content (%) ≥ 42.65%, the sample compared with the reference genome, the total mapping
- PCA analysis of gene expression values (FPKM) of all samples (Figure 6a) shows high
- variability of samples between groups and good parallel correlation of samples within
- homogenize expression data rows. Genes or samples with similar expression patterns are
- is the second principal component). (b) Venn diagram of differential genes in different samples.
- (c) Heat map of differential gene clustering. (Horizontal coordinates are sample names, and vertical
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Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.