Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- in extrusion efficiency across MMC-treated oocytes (86.83 ± 1.87% vs. control), manifesting
- as: 84.68 ± 1.00% (0.2 µM, p > 0.05), 67.92 ± 2.08% (1 µM, p < 0.01), and 33.08 ± 0.98%
- n = 81; 5 μM MMC, n = 91. (D) mRNA expression levels of TNFAIP6, PTGS2, PTX3, and HAS2 were
- groups. Control, n = 81; MMC, n = 83. (H) Abnormal chromosome arrangement rate of oocytespin< 0.01, and
- control group and MMC group. Control, n = 88; MMC, n= 81. Scale bar = 20 μm. (G) CAT repre-
- and MMC group. Control, n = 88; MMC, n= 81. Scale bar = 20 µm. (G) CAT representative images
- Control, n = 50; MMC, n = 50. Scale bar = 0.5 µm. (S) Relative level of ATP in control group and
- = 77; MMC, n = 80. Scale bar= 20 μm. (E) Representative images of LC3 in the control and MMC
- intensity of P62 in control group and MMC group. Control, n = 77; MMC, n = 80. Scale bar = 20 µm.
- n = 82. (I) Representative images of Parkin in the control and MMC groups. Scale bar = 20 µm.
- protein levels of Pink1, Parkin and P62 were quantitatively analyzed (n = 3). (M) Representative
- analysis of p-AMPK and AMPK protein levels (n = 3). (Q) p-mTOR, mTOR relative protein level.
- (R) Quantitative analysis of p-mTOR and mTOR protein levels (n = 3). (S) Representative images of
- P62. Scale bar = 20 µm. (T) The relative fluorescence intensity of P62. Control, n = 97; MMC, n = 90;
- MMC + MHY1485, n = 95. Scale bar = 20 µm. (U) Representative images of LC3. Scale bar = 20 µm.
- decreased maturation rate of oocytes induced by MMC (Control: 88.44 ± 0.86% vs. 10 µM
- MT: 83.52 ± 2%; p > 0.05). In addition, 10 µM MT treatment also significantly decreased ROS
- n = 90. (I) group. Scale bar
- Transmission = 100 microscope
- arrowheads represents the site of mitochondrial damage. Control, n = 50; MMC, n = 53; MMC + MT,
- ovaries (n = 5). (I) H&E staining representative images of ovary of mice in control
- morphology and chromosome arrangement in the control group and the MMC group. Scale bar = 5
- 86; MMC, n = 83. (E) Abnormal chromosome arrangement rate of oocytes in control group and
- phology and chromosome arrangement in the control group and the MMC group. Scale bar = 5 µm.
- (D) Spindle abnormality rate of oocyte in control group and MMC-treated group. Control, n = 86;
- MMC, n = 83. (E) Abnormal chromosome arrangement rate of oocytes in control group and MMC
- n = 101; MMC, n = 108. (P) Representative images of mitochondrial autophagy in control group and
- assembly and misaligned chromosomes. A previous study showed that MMC (1 mg/kg/d)
- increased LC3 levels indicated that MMC treatment promoted autophagic flux. Meanwhile,
- in sterile saline (0.9% NaCl) supplemented with antibiotics, and transported to the labo-
- supplementation (75 mg/L penicillin and 50 mg/L streptomycin) to eliminate surface
- (TL-HEPES-PVA) solution (0.1% polyvinyl alcohol), and the pelleted cellular fraction was
- (38.5 ◦ C, 5% CO2 , saturated humidity). The IVM protocol employed a TCM-199-based
- (10 ng/mL EGF), and biological supplement (10% v/v autologous porcine follicular fluid).
- were subjected to 0.1% ammonium hydroxide solution or Scott’s sodium bicarbonate-
- deionized H2 O. Subsequent cytoplasmic counterstaining was achieved using a 0.5% eosin Y
- an ascending ethanol gradient (70% → 80% → 90% → absolute ethanol), with each step
- COCs into a phosphate-buffered saline (PBS) medium containing 0.1% hyaluronidase and
- cumulus–oocyte complexes underwent enzymatic denudation using 0.2% hyaluronidase.
- Oocytes were initially immobilized in a 2.5% glutaraldehyde solution at 4 ◦ C for
- 2 days. They were then treated with a 1% agar solution for 40 min, followed by a gradual
- with PBS-polyvinylpyrrolidone (PVP), fixed with 4% paraformaldehyde for 30 min, and
Methods (brief)
- samples underwent four cycles of lavage using physiological saline containing antibiotic
- injections, blood and ovarian tissues were collected. Blood samples were collected after
- anesthesia, and serum was obtained through centrifugation. Each serum sample volume
- GV stage oocytes were collected. GV oocytes were cultured for 16 h in the M2 medium.
- MD01) for mitophagy flux analysis. The fluorescent probes were incubated with samples
- microscope was applied for sample examination and imaging.
- addition of 8 µL 5 mM Tris-HCl buffer (pH 8.0). Samples were subjected to thermal
- enzymatic digestion at 55 ◦ C for 30 min. Enzyme inactivation was achieved through
- lucida removal through pronase digestion (0.5% w/v). Following three successive washes in
- 4.15.2. Trypsin Digestion
-
- Bjørklund, G.; Dadar, M.; Mutter, J.; Aaseth, J. The toxicology of mercury: Current research and emerging trends. Environ. Res.
-
- Nguyen, T.N.; Padman, B.S.; Lazarou, M. Deciphering the Molecular Signals of PINK1/Parkin Mitophagy. Trends Cell Biol. 2016,
Implications
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Verification notes
- Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing
wiki/sources/pages before creation. - Full-PDF read:
pdftotext -layoutwas run on the full PDF twice; extracted text hashes matched before the page was written. - Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
- Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
- HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.
Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.