Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- (Baker et al., 2012; Munday et al., 2014). Deletions and chimeric rearrangements between TbAQP2
- Table 1. Cryo-EM data collection, refinement, and validation statistics.
- Allowed (%) 3.31 3.72 3.72
- Disallowed (%) 0 0 0.41
- 33% amino acid sequence identity). The overall arrangement of α-helices in TbAQP2 is identical to
- stable (Figure 5—figure supplement 2a, b). In contrast, water shows a different pattern of high occu-
- attributable to residual uptake of the compound through the TbAT1/P2 transporter (Carter and Fairlamb, 1993; de Koning et al., 2000).
- a >90% decrease in pentamidine uptake and in many cases no residual pentamidine transport was
- using potential of mean force (PMF) calculations with umbrella sampling. The data for WT TbAQP2
- in cold homogenisation buffer (100 mM NaCl, 20 mM HEPES pH 7.5, 5% (vol/vol) glycerol, 2 mM
- in a Potter-Elvehjem glass homogeniser on ice. Membranes were solubilised using 1% (wt/vol) final
- concentration n-dodecyl-β-d-maltopyranoside (DDM, Anatrace) and a final concentration of 0.03%
- washed three times with 20 column volumes washing buffer (100 mM NaCl, 20 mM HEPES pH 7.5, 2%
- (vol/vol) glycerol, 2 mM PMSF, 1× Complete Protease Inhibitor Cocktail (Sigma), 0.05% (wt/vol) DDM,
- 0.03% (wt/vol) CHS, 100 mM imidazole) and was eluted twice with one column volume elution buffer
- (100 mM NaCl, 20 mM HEPES pH 7.5, 2% (vol/vol) glycerol, 1× Complete Protease Inhibitor Cocktail
- Mark IV (95% relative humidity, 4°C). All cryo-EM datasets were collected on Titan Krios microscopes
- sure time of 60 s resulting in a total exposure of (30 e/pixel) and a target defocus range of –1.4 and
- in a total exposure of 54 and 57.5 e/Å2 with target defocus ranges of –1.5 and –3.3 µm, and –1.2 and
- a range of sharpening and blurring options using CCPEM (Wood et al., 2015; Burnley et al., 2017).
- with a coupling constant of 5 ps (Bussi et al., 2007; Parrinello and Rahman, 1981). Short-range van
- for long-range Lennard–Jones interactions (Darden et al., 1993). Bonds containing hydrogen were
- Chien CTH, Helfinger LR, Bostock MJ, Solt A, Tan YL, Nietlispach D. 2017. An adaptable phospholipid
Methods (brief)
- Sample size was not predetermined by statistical methods, no randomisation was applied to experi-
- plasma cannot alter the channel’s structure). The cells were then collected by centrifugation (10 min,
- ugal filters (100,000 MWCO; Merck) to final concentrations between 0.8 and 1.6 mg/ml. Final samples
- Vitrified sample preparation and data collection
- Mark IV (95% relative humidity, 4°C). All cryo-EM datasets were collected on Titan Krios microscopes
- (Thermo Fisher Scientific) operating at 300 kV. SapA-TbAQP2-NP with glycerol was collected at the
- –3.2 µm. Datasets of SapA-TbAQP2-NP with bound pentamidine or melarsoprol were collected at the
- editing, Cloned, overexpressed, purified, and analysed protein samples; Marzuq Ungogo, Investi-
- Investigation, Writing – review and editing, Prepared grids, screened and collected data; Pablo
- Castro-Hartmann, Investigation, Writing – review and editing, Prepared grids, screened and collected
- collected data; Sundeep Chaitanya Vedithi, Investigation, Writing – review and editing, Performed
- pressed, purified and analysed protein samples and managed the overall project
Implications
This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.
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Verification notes
- Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing
wiki/sources/pages before creation. - Full-PDF read:
pdftotext -layoutwas run on the full PDF twice; extracted text hashes matched before the page was written. - Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
- Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
- HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.
Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.