Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- J.C.; Aboueissa, A.E.-M.; Wise, J.P., Sr. at the highest concentration, securin protein levels were decreased to 15.3% of control cells, while
- Hexavalent Chromium Targets securin mRNA quantification was 7.9% relative to control cells. Additionally, loss of securin function
- 170 kDa protein fragment to increase to 200%, 310%, and 380% of control
- 170 kDa protein fragment to increase to 200%, 310%, and 380% of control levels with 0.1, levels with 0.1, 0.2,
- ments of 70increased to 220%,
- kDa increased 420%,420%,
- Full-length separase levels increased to 115.4%, 157.7%, and 171.5%
- 4D). Full-length separase levels increased to 115.4%, 157.7%, and 171.5% above control above control values,
- mechanisms in theand 12.7%
- levels were reduced to 43.7%, 24.5%, and 12.7% of control values (Figure 7). Thesecells.
- G2/M. Data are expressed as percent of untreated control cells and reflect the mean of three
- G2/M. Data are expressed as percent of untreated control cells and reflect the mean of three or four or four
- from Lonza (Allendale, NJ, USA). Trypsin-EDTA (0.25%) and KaryoMAX® Colcemid So-
- lution (10 ug/mL) were purchased from Gibco. Zinc chromate (CAS# 13530-65-9, 99.7%
- described (54). This clonal cell line has an hTERT-extended lifespan with a normal, stable
- adherent, subconfluent layer in DMEM/F-12 media, supplemented with 15% cosmic calf
- released from dishes by incubation with 0.25% trypsin-EDTA. Cells were collected, washed
- with 0.25% trypsin-EDTA. Cells were collected and washed with PBS. Total RNA was
-
- Relative quantification value of 10 means the gene is 10 times more expressed, while a
- value of 0.1 means 10 times less expressed.
- analysis of all cells. Adherent cells were released from dishes with 0.25% trypsin-EDTA
- Cells were fixed in 4% PFA (paraformaldehyde) on ice for 15 min. Cells were pelleted by
- PBS and combined with 70% ethanol, added dropwise during gentle vortexing. Fixed cells
- were centrifuged, resuspended in 1 mL 70% ethanol, and stored at −20 ◦ C until analysis.
- and Heritable Numerical and Structural Changes and a DNA Repair-Deficient Phenotype. Cancer Res. 2018, 78, 4203–4214.
-
- Levy, L.S.; Martin, P.A.; Bidstrup, P.L. Investigation of the Potential Carcinogenicity of a Range of Chromium Containing Materials
Methods (brief)
- used as aas a loading
- tein sample loading buffer, Odyssey blocking buffer, IRDye® 800CW, and IRDye® 680RD
- released from dishes by incubation with 0.25% trypsin-EDTA. Cells were collected, washed
- centrifuged 10 min at 14,000 rpm and supernatant was collected and prepared with 4X
- protein sample loading buffer. Protein samples in loading buffer were heated for 10 min at
- 70 ◦ C and stored at −20 ◦ C. Prepared sample volumes equivalent to 75,000–81,000 lysed
- was isolated and stored on ice until preparation with 4X protein sample loading buffer
- as for whole cell protein samples. The nuclear fraction was resuspended in NER (nuclear
- time of 40 min. Samples were centrifuged at 14,000 RPM and the nuclear fraction was
- prepared with 4X protein sample loading buffer as for whole cell protein samples and
- with 0.25% trypsin-EDTA. Cells were collected and washed with PBS. Total RNA was
- instrument. Samples were stored at −80 ◦ C.
- reaction, was added to the master mix for each sample. Control samples with no reverse
- (∆Ct = Ct gene target—Ct endogenous control; ∆∆Ct = ∆Ct sample 1—∆Ct calibrator
- the end of the treatment time, the media were collected in 50 mL conical tubes to ensure
- and collected and combined with harvested media. Cells were centrifuged to a pellet at
- each sample, 200,000 cells were analyzed. Flow cytometry data were analyzed using the
-
- Beaumont, J.J.; Sedman, R.M.; Reynolds, S.D.; Sherman, C.D.; Li, L.H.; Howd, R.A.; Sandy, M.S.; Zeise, L.; Alexeeff, G.V. Cancer
Implications
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Verification notes
- Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing
wiki/sources/pages before creation. - Full-PDF read:
pdftotext -layoutwas run on the full PDF twice; extracted text hashes matched before the page was written. - Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
- Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
- HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.
Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.