Quantitative Proteomic Analysis of Cyanide and Mercury

Overview

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Key numbers

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• affecting the fetus (1, 2, 14). Mercury is associated with a broad range of severe diseases,
• revealed that the overall number of identified proteins (2,689) represented about 60%
• CN 1 Hg and CN proteomes (see Table S1 in the supplemental material). In this com-
• were found differentially expressed (see Fig. S4 in the supplemental material; Table
• proteome (Table S1). The gene ontology (GO) analysis (27) using the comparative
• sented in the presence of Hg (Fig. 3; Table S1). Among proteins highly overrepresented
• over- or downrepresented in the CN 1 Hg proteome (Table 1). Genes induced in the
• (Table 1). The expression of the nitC gene coding for the nitrilase NitC that is essential
• nitC gene (Table 1).
• Table S2 in the supplemental material).
• TABLE 1 Transcriptional expression analysis by qRT-PCR of some P. pseudoalcaligenes CECT 5344 genes encoding proteins affected by
• proteins found exclusively under the CN 1 Hg condition (Fig. S4; Table S1). Among the
• MerP1 and MerP3 (Fig. 3; Table S1). MerP and MerT proteins have been described to be
• one S-transferase was found induced in the presence or mercury (Fig. 3; Table S1). This
• glutathione peroxidase (Fig. 3; Table S1) are involved in redox reactions and protection
• was exclusive of the CN 1 Hg proteome (Fig. 3; Table S1). Thioesterases catalyze the
• was found exclusively in the CN 1 Hg proteome (Fig. 3; Table S1). Recently, it has been
• Table S1). Most redox signals are recognized by cysteine residues, which can be oxi-
• was overrepresented in the presence of mercury (Fig. 3; Table S1). It has been
• a- and b -subunits of the ribonucleoside-diphosphate reductase (Fig. 3; Table S1). The
• the ribosome-recycling factor RRF (Table S1), suggesting that general cellular processes
• and the heme/Fe-S containing bacterioferritin (Fig. 3; Table S1). The formation of com-
• transaminase, and D-amino acid dehydrogenase (Fig. 3; Table S1). Cysteine desulfurase
• mercury reductase, and the coregulator MerD. Thus, the strain CECT 5344 is a suitable can-
• out in this work (Fig. 3 and 6A; Table S1). Additionally, the merR2 gene was identified
• (Fig. 3; Table S1). PhoR is a transcriptional regulator that activates pho gene expression,
• Hg proteome of this bacterium (Fig. 3; Table S1), including the thioredoxin-dependent
• Pellets with cells were washed with 1 mL of a solution containing 0.85% NaCl. Cells were heated at 80°C
• for 96 h, and cell dry weight was determined. Pellets were digested in 69% HNO3 (trace-metal grade;
• cells were resuspended in 300 m L of a lysis buffer, containing 8 M urea, 50 mM Tris-HCl (pH 7.5), 4% 3-((3-
• cholamidopropyl)-dimethylammonio)-1-propanesulfonate (CHAPS), and 1% SDS. Samples were disrupted
• previously (63). Oligonucleotides sequences used as primers (see Table S3 in the supplemental material)
• doi.org/10.1007/s11356-016-7968-3. mercury in humans: methods, measurements and meaning. Biochim Bio-

Methods (brief)

• phy-tandem mass spectrometry (LC-MS/MS) has been applied to unravel the molecular ba-
• characterized through liquid chromatography-tandem mass spectrometry (LC-MS/MS),
• was determined by inductively coupled plasma-mass spectrometry (ICP-MS) from CECT
• intracellular determinations of mercury concentration by ICP-MS in the CECT 5344 cells
• biological samples were analyzed. Cells were centrifuged (10,000 rpm, 10 min, and 4°C) at 7.5 h of
• Fisher). Mercury concentration was determined by inductively coupled plasma-mass-spectrometry (ICP-
• cholamidopropyl)-dimethylammonio)-1-propanesulfonate (CHAPS), and 1% SDS. Samples were disrupted
• by qRT-PCR using three biological samples, each with two technical replicates. RNA isolation was per-
• for qRT-PCR. A pair of samples were considered significantly different when the adjusted P value was
• 43. Bjørklund G, Crisponi G, Nurchi VM, Cappai R, Djordjevic AB, Aaseth J.

Implications

This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.

Wiki pages this source may touch

• Fish — marine, non-predatory (sardines, anchovies, salmon, cod)
• Mercury
• Mercury
• Cadmium
• Arsenic
• Nickel

Verification notes

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Update history

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