published maps and institutional affil- concentrated in animal tissue through a cation/carnitine transporter protein known as

Overview

This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.

Key numbers

The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:

• waukee, WI, USA). Deuterium oxide (D, 99.9%) was purchased from Cambridge Isotope
• Laboratories (Andover, MA, USA). Hydrogen peroxide (30% in water) was purchased
• in a bright orange solution. After 6 min, free-base Cys (24.3 g, 200.4 mmol, 3.0 eq.) was
• 6.5 with 30% ammonia hydroxide while nitrogen was being bubbled through the solution
• were diluted 10-fold with a 50 mM ammonium bicarbonate, 0.1% formic acid solution, then
• The scan parameters were as follows: scan range, 70–1500 m/z; resolution, 70,000; polarity,
• Singlet oxygen was produced using the photosensitizer rose bengal in a 90% D2 O
• in 20 mM potassium phosphate-buffered 90% D2 O, pH 7.0. To both samples, 50 µM RNO
• Holliston, MA, USA) at 5 µL/min into an isocratic (50% water and 50% acetonitrile with
• 0.1% formic acid) mobile phase flow from a Shimadzu Prominence high-performance liquid
• on a sample with 1 mM EGT and 10 µM rose bengal in 90% D2 O, irradiated with light for
• mass range was altered to m/z 100 to 600. A control experiment was run using the same
• in the presence of EGT and H2 O2 in a 2:1 ratio and is stable for at least several hours. This
• pH in the presence of EGT and H2O2 in a 2:1 ratio and is stable for at least several hours.
• consumption of NADPH after about 3.5 min. This activity is equivalent to ~20% of the
• derived values of KM and kcat in Table 1 are meaningless.
• charged trimethylated amine group hosting a very stable cation (49–51). Intracellular stores
• of EGT are typically kept in the low millimolar range (1,5,53). A reduction in activity
• higher than reported in Table 1 because MS analysis of the oxidation of EGT with H2 O2
• (Figure 2) revealed the presence of hercynine. This means that the calculated amount of
• bengal in the presence of a visible light source in 90% D2 O for 20 min. The decision to
• bengal in the presence of a visible light source in 90% D2O for 20 min. The decision to use
• Table 2. Kinetic parameters of Sec-TrxR with 2-thioHis and EGT oxidized with 1O2 as substrates.
• Table 2. Kinetic parameters of Sec-TrxR with 2-thioHis and EGT oxidized with 1 O2 as substrates.
• were stable was to run to verify that 5-oxo-EGT,
• The results of these assays are summarized in Table 3. The truncated enzyme TrxR∆3
• between Sec-TrxR and TrxR∆3 was 99.8–99.9%, and activity showed little dependence upon
• was 98.4–99.1% lower than Sec-TrxR. DmTrxR had more activity than either of the Sec-TrxR
• The activity of DmTrxR toward ESSE was 94–97% lower than Sec-TrxR. Sec-TrxR showed the

Methods (brief)

• solutions were obtained to determine what oxidized species were present. The samples
• was a Chemyx Fusion 101 (Stafford, TX, USA). For each sample, 12–14 scans were averaged.
• One NMR sample was prepared with 10 mM EGT in D2 O, and one was prepared with
• Advance III HD 500 MHz NMR spectrometer for both samples. To obtain quantitative
• H2 O2 was added to the second sample exactly 10 min before recording NMR spectra.
• 1 O using the procedure from Herman and Neal and Kraljić and El Mohsni (40,41). Samples
• in 20 mM potassium phosphate-buffered 90% D2 O, pH 7.0. To both samples, 50 µM RNO
• was added. An initial absorbance scan of both samples was taken, noting the absorbance of
• RNO at 440 nm. Both samples were then incubated under light, and absorbance scans were
• obtained every 3–4 min for 10 min. In addition, samples of 1 mM 2-thioHis with 10 µM rose
• MS analysis of the irradiated rose bengal/2-thioHis and rose bengal/EGT samples
• 2-thioHis and EGT samples with rose bengal in D2 O was completed within 30–45 min of
• irradiating the samples to avoid significant decomposition of any oxidation products. For
• (ESI) was used as the ionization source. Samples were diluted with deionized water and
• chromatography (HPLC) system (Shimadzu Scientific Instruments, Columbia, MD, USA).
• room temperature, respectively. A 1 mL sample was prepared with 10 µM rose bengal and
• 1 mM EGT or 2-thioHis in D2 O in a small glass test tube. This sample was incubated under
• on a sample with 1 mM EGT and 10 µM rose bengal in 90% D2 O, irradiated with light for

Implications

This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.

Wiki pages this source may touch

• Fish — marine, predatory (tuna, swordfish, shark, king mackerel)
• Mercury
• Mercury

Verification notes

• Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing wiki/sources/ pages before creation.
• Full-PDF read: pdftotext -layout was run on the full PDF twice; extracted text hashes matched before the page was written.
• Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
• Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
• HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.

Update history

The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.