Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- greater concern to the ecosystem and public health due to 2021). Spinacia oleracea is a worldwide cultivated vegetable
- et al., 2022a). NaHS also promotes photosynthesis in Hordeum temperature of 35/40◦ C and day/ night humidity of 60/70%.
- under Cr stress. Therefore, this study aimed to ascertain the properties are given in Supplementary Table 1. The soil sample
- were carefully washed and sterilized in 0.1% HgCl2 solution the plant was determined by measuring the weight of the plant
- water to collect the exudates from the root surface. The samples 0.1% titanium sulfate in 20% (v/v) H2 SO4 and centrifuged at
- sample was extracted with 8 mL of 95% acetone for 24 h at 4◦ C in
- phosphate buffer solution (pH 7.8) containing 1% polyethene as the substrate. A reaction mixture (3 mL) containing 0.05 mL
- 25 mM potassium phosphate buffer with 2 mM EDTA (pH 7.0) freeze-dried exudates were mixed with ethanol (80%), and 20 µL
- homogenized with 10 mL of ethanol (80%) and filtered through at 214 nm wavelength with the help of a detector (UV–VIS
- aqueous sulfosalicylic acid (3%). The mixture was centrifuged at containing 10 mL of HNO3- HClO4 (3:1, v:v); this mixture
- grown under different levels of Cr (0 (no Cr), 150, and 300 µM) in sandy loam soil. Values in the figures indicate just one harvest. Mean ± SD (n =
- 4). Two-way ANOVA was performed and mean differences were tested by HSD (P < 0.05). Different lowercase letters on the error bars indicate
- the figures indicate just one harvest. Mean ± SD (n = 4). Two-way ANOVA was performed and mean differences were tested by HSD (P < 0.05).
- indicate just one harvest. Mean ± SD (n = 4). Two-way ANOVA was performed and mean differences were tested by HSD (P < 0.05). Different
- also noticed that Cr toxicity did not significantly affect the (H2 O2 ) initiation, and electrolyte leakage (%) increased
- figures indicate just one harvest. Mean ± SD (n = 4). Two-way ANOVA was performed and mean differences were tested by HSD (P < 0.05).
- observed that the contents of MDA, H2 O2 , and EL (%) were application of SNP and NaHS.
- levels of Cr (0 (no Cr), 150, and 300 µM) in sandy loam soil. Values in the figures indicate just one harvest. Mean ± SD (n = 4). Two-way ANOVA
- was performed and mean differences were tested by HSD (P < 0.05). Different lowercase letters on the error bars indicate significant differences
- loam soil. Values in the figures indicate just one harvest. Mean ± SD (n = 4). Two-way ANOVA was performed and mean differences were tested
- 300 µM) in sandy loam soil. Values in the figures indicate just one harvest. Mean ± SD (n = 4). Two-way ANOVA was performed and mean
Methods (brief)
- signaling molecules may improve growth and grain yield in S. used for this experiment was collected from the experimental
- under Cr stress. Therefore, this study aimed to ascertain the properties are given in Supplementary Table 1. The soil sample
- The mature and healthy seeds of spinach (Spinacia oleracea and root samples from each treatment group were picked after
- Pakistan)) were collected from the Ayub Agricultural Research The leaves were washed with distilled water, placed in liquid
- from the rhizoboxes, and the roots were washed with redistilled and leaf), 3 mL of sample extract was mixed with 1 mL of
- water to collect the exudates from the root surface. The samples 0.1% titanium sulfate in 20% (v/v) H2 SO4 and centrifuged at
- collected in Eppendorf tubes (Greger and Landberg, 2008). The evaluated at 410 nm. The H2 O2 level was computed by the
- collected samples were mixed with NaOH (0.01 M) to analyze extinction coefficient of 0.28 mmol−1 cm−1 . The content of
- the organic acids. However, the samples used for the analysis of H2 O2 was measured by the method presented previously (Jana
- conductivity (EC1 ). The samples were autoclaved at 121◦ C for
- Leaves were collected for the determination of chlorophyll
- sample was extracted with 8 mL of 95% acetone for 24 h at 4◦ C in
- Determination of oxidative stress sample was measured by using a spectrophotometer (xMarkTM
- and 2.8 mL of 50 mM phosphate buffer with 2 mM ETDA (pH roots and shoots were analyzed by using the atomic absorption
- 7.0). The CAT activity was measured based on the decline spectrophotometer (AAS) model Agilent 240FS-AA.
- high-performance liquid chromatography (HPLC), having a
- Flexer FX-10 UHPLC isocratic pump (PerkinElmer, MA, USA).
- Determination of non-enzymatic The mobile phase used in HPLC comprised an acidic solution
Implications
This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.
Wiki pages this source may touch
- Root-Vegetable Purees
- Baby Sunscreen, Mineral (ZnO + TiO2)
- Mercury
- Cadmium
- Arsenic
- Nickel
- Aluminum
- Chromium
Verification notes
- Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing
wiki/sources/pages before creation. - Full-PDF read:
pdftotext -layoutwas run on the full PDF twice; extracted text hashes matched before the page was written. - Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
- Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
- HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.
Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.