Overview
This source page is a mechanical bulk-ingest record for a PDF in the methylmercury infant-formula research pull. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
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- ISSN 1678-4324 Online Edition
- 1,2Orissa University of Agriculture and Technology, College of Agriculture, Department of Agricultural Biotechnology,
- Both Si transporters (Lsi1and Lsi2) were up-regulated under salinity stress.
- Lsi1 transporter is concerned with maintaining osmotic balance, while Lsi2 transporter is
- Lsi2 expression was found to be highly variable among rice genotypes.
- accumulator) which differs in Si uptake potential under saline (10ds/m EC) and non- saline conditions were
- varied in both cultivars, due to their differential Si-accumulation. Molecular characterizations of Lsi1 protein
- revealed its involvement in the movement of ion and water and therefore prevent osmotic stress. The Lsi2 is
- of Lsi2 provides a possible explanation for differential genotypic uptake of silicon.
- Keywords: differential Si-uptake; indicarice; Lsi1 and Lsi2 gene expression; Silicon; salt tolerance; qRT
- development of plants. Around 20% of total cultivated land and 33% of irrigated agricultural lands are affected
- by high salinity (1). In India, 6.74 million ha area is adversely affected by salinity resulting in a decline in
- production and productivity (2). Salinity occurs as a result of nutritional toxicity, water stress and accumulation
- of excess sodium ions (Na+) in the plant species (3). The salinity also hampers photosynthesis/carbohydrate
- in oxidative stress in plants (4,5). The essential staple food of Asia, ‘Rice’, is highly sensitive to salinity. The
- rice yields decrease up to 12% per unit increase above 3.0 dS/m in electrical conductivity (EC) (6). For dealing
- stability of cell membranes (13). Si increase stress tolerance and decrease membrane damage in tomato
- transpiration rate, stomatal conductance of stress-induced plants (16). In rice, highest silicon accumulation
- up to the level of 10% of shoot dry weight was reported by various authors, so the trait could be exploited for
- building better salt tolerant genotypes (7,17). In Japonica rice, two silicon transporters were reported to be
- associated with the uptake of silicon from the soil solution to the apoplast, i.e. Lsi1 and Lsi2, respectively
- (18). Based on beneficial effect of silicon in rice, the present study focused on evaluating the change occurring
- stage. The salt-tolerant variety ‘Lunishree’ and the salt-sensitive ‘IR29’ were used as standard check cultivars
- in salt screening. Seeds of all four cultivars were surface sterilized with 0.1% (w/v) mercury chloride for two
- minutes and 0.5% bavistin (w/v) for fifteen minutes and were allowed to germinate in dark at 28 ̊C for five
- containing Yoshida Nutrient Solution (YNS) and were exposed to light (3000 lux) for 12 h photoperiod with
- 25 ̊C day/night temperature (19). After two weeks, the planted nylon net frames of bowls were finally fitted
- into the styrofoam boards. The styrofoam boards were allowed to float on plastic pots filled with 3 litre of
- Yoshida culture solution (pH-5.0±0.5). These pots were then placed in a glasshouse maintained at 28±2 ̊C
- with 50% relative humidity. The culture solution was changed at four- day intervals and EC was maintained.
- electrical conductivity (EC) of 10ds/m (~100mM), while the non- saline serving as control showed EC of
- 1ds/m. Thus, the experiment was designed in completely randomized design (CRD) as control without Si and
-
- NaCl (10ds/m)-T4. The young expanded leaves were harvested after 7 days of saline treatment and stored
- at -20 ̊C prior to analysis. Measurements on sodium and potassium content, electrolyte leakage percentage
- Role of Si transporter genes in salinity stress 3
- The shoots/roots were dried at 70 ̊C for 5 days and ground with liquid nitrogen into a fine powder.
- Aminomolybdate method was used to determine the Si content in rice (20). The ground samples of leaves
- (0.1 g) were digested with 3mL of 50% NaOH in a volumetric flask. About 1mL of digested sample solution
- was transferred to a 50mL polyethylene tube. Further, 30mL of 20% acetic acid and 10mL ammonium
- molybdate (54 g/L, pH 7.0) were also added. The solution was shaken thoroughly and kept for 5min and then
- added 5mL of 20% tartaric acid and 1mL reducing solution. The volume was made up to 50mL with 20%
- acetic acid. The absorbance was taken at 650 nm using UV/Vis-Spectrophotometer (LAMBDA 365, Perkin
Methods (brief)
- collected from Rice Research Station of OUAT, Odisha. These genotypes having differential Si-uptake
- Aminomolybdate method was used to determine the Si content in rice (20). The ground samples of leaves
- (0.1 g) were digested with 3mL of 50% NaOH in a volumetric flask. About 1mL of digested sample solution
- Sodium and potassium content in leaves was determined (21). About 10 mg dried leaves of each sample
- The samples were diluted to 10 times. The elemental analysis was made by atomic absorption
- spectrophotometer (iCE™ 3300 AAS).
- hours, the initial electrical conductivity of the samples (EC1) was measured. The tubes were later autoclaved
- at 121 °C for 20 minutes and cooled to 25 °C. Finally electrical conductivity of each sample (EC 2) was
- was used to determine the total protein content in the samples utilizing bovine serum albumin as the standard
- (23).The reaction mixture consists of a total volume of 4ml containing 1mLof sample extract, 3mLof 2% (w/v)
- ORF (Open Reading Frame) finder encoded the presence of 117aas in genotype Badami and 102aas in
- acid into a single continuous amino acid sequence of 208aas. The NCBI Conserve Domains hit showed high
- samples. The data obtained were analyzed in a most common model of 2-ΔΔCt method (38). The real time
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