Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- directly promote the expression of a range of detoxification the peroxiredoxin Tsa1 have a shortened replicative lifespan although,
- treated with prdx-2 RNAi. The effect of prdx-2 RNAi was examined on Nevertheless, 30% of prdx-2-mutant animals expressing DAF-28::GFP
- transgenic lines containing arrays expressing different wild-type SKN- lacked any GFP-positive coelomocytes and only 35% of prdx-2-mutant
- 1::GFP isoforms. SKN-1B/C::GFP encodes both the SKN-1B and SKN- animals contained more than one coelomocyte with detectable GFP flu-
- (Tullet et al., 2008). Nuclear levels of SKN-1::GFP are barely detectable were easily detected in every wild-type DAF-28::GFP animal (Fig. 2A).
- mutant animals (Fig. 3B and Table S1, Supporting information). A sod-
- explain the increased arsenite resistance associated with loss of PRDX-2, dauer even when food is plentiful (Table 1) (Henderson & Johnson,
- active form of the insulin receptor (DAF-2), the function of which is (Table 1), indicating that PRDX-2 is not required for dauer formation.
- daf-2 (e1370) animals were hyper-resistant to arsenite-induced stress conditions at 15, 20 or 25 °C (data not shown and Table 1). At
- (gk169), did not cause a further increase in the expression of a sod-3p:: formation (< 1%), even when food is plentiful (Ailion & Thomas, 2000).
- (A) Wild type (N2) DAF-28::GFP (n = 45) Fig. 2 PRDX-2 is required for insulin (DAF-28::GFP) secretion under well-fed
- 10 identical exposures. Error bars represent the standard deviation from the mean. n
- (Olahova et al., 2008) (Fig. 5, Table S2). Indeed, although daf-2 (e1370)-
- 0.5 n = 37 n = 30 promoting activity of nuclear DAF-16 at 15 °C or, alternatively, acted
- dauers formed by daf-2 (e1370) mutants at 20 °C (Table 1). Further- The transcription factor, heat-shock factor, HSF-1, coregulates many
- experiments and statistical analysis, see Table S1. (C, D) The survival of L4 larval stage wild-type (N2), daf-16 (mu86)- and skn-1 (zu67)-mutant animals microinjected with
- Table 1 PRDX-2 is not required for the constitutive dauer phenotype associated with reduced DAF-2 activity and slightly suppresses dauer formation in daf-2 (e1370) mutants at
- three independent experiments. The increase in % dauer formation in prdx-2 (gk169) daf-2 (e1370) compared with daf-2 (e1370) was not statistically significant (chi-square test
- P = 0.155). The increase in % dauer formation in prdx-2 RNAi-treated compared with vector control-treated akt-1 (mg306)-mutant animals was statistically significant (chi-
- analysis, see Table S2.
- (IPTG) was added to the cultures used to seed RNAi plates (0.3% (w/v)
- NaCl, 1.7% (w/v) agarose, 0.25% (w/v) bactopeptone, 0.08% (w/v) yeast
- Animals were mounted on a 3% agarose pad, anaesthetized with survival test (MINITAB 16) was used to determine whether differences
- 0.06% levamisole, unless otherwise indicated, and microscopic obser- between groups were statistically significant.
- indicated temperatures (Table 1) for 48 h before dauer formation was
- statistically significant differences between groups. mounted onto microscope slides containing 2% agarose and anesthetized
- with 0.06% levamisole. All images were taken under the 639 objective
- SKN-1B/CS393A::GFP or SKN-1opS12A::GFP, approximately ten late L4 larval AXIOVISION 3.1.2.1 software. A defined area ( 10%) of a coelomocyte was
- stage animals were transferred to fresh RNAi plates. The F1 progeny outlined in each animal, and the mean pixel value and standard error were
- Author contributions Iraqui I, Kienda G, Soeur J, Faye G, Baldacci G, Kolodner RD, Huang ME (2009)
- pathways in C. elegans. Aging Cell 12, 1073–1081. Table S2 Statistical analysis of Lifespan data for experiments shown in Fig. 5.
Methods (brief)
- prepared RNA samples. Each panel depicts the levels of a particular mRNA in prdx-2 mutant normalized to wild-type (N2). Error bars represent the SEM. For individual
- scored as dead and removed from the plate. Log-rank survival analysis The RNA samples were DNaseI treated (Ambion and PrimerDesign Life
- Author contributions Iraqui I, Kienda G, Soeur J, Faye G, Baldacci G, Kolodner RD, Huang ME (2009)
Implications
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Verification notes
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Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.