methylmercury toxicity

Overview

This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.

Key numbers

The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:

• conditions was 10% or less (data not shown). Measurement of the transport of radioactive pyruvate into isolated
• wavelength of 530 nm. Subsequently, the HBSS was replaced with DMEM containing 10% Alamar blue solution.
• pyruvate dehydrogenase. In this approach, treatment with methylmercury caused only an ~10% decrease in the
• chondria from Yil006-deleted yeast were approximately 30% of those in wild-type yeast, indicating that mito-
• Each fraction was lysed by sonication for 30 sec (output 4, duty 30%, Branson Sonifier 450). The lysate was filtered
• Modified Eagle’s Medium (DMEM) (Sigma, St. Louis, MO, USA) supplemented with 10% (v/v) foetal bovine
• serum (Bio-west, Kansas City, MO, USA), 0.06% L-glutamine, and 100 U/mL penicillin (Invitrogen, Grand
• Island, NY, USA) at 37 °C in a humidified incubator containing 5% (v/v) CO2. IMR-32 cells (2 × 104 cells/well)
• cated concentrations for 24 hr. Cell viability was measured using a 10% Alamar blue solution using an excitation

Methods (brief)

• was approximately 1.7 (1011 cells/L). The yeast cells were collected and incubated in 60 mL of Tris buffer (0.1 M
• spheroplasting buffer (1.2 M sorbitol, 20 mM KPi, pH 7.4), collected by centrifugation (2,000 × g for 5 min) and
• After centrifugation at 1,500 × g for 5 min, the supernatant was collected and centrifuged at 12,000 × g for 10 min,
• in the dark. Rhodamine 123-treated cells were collected with 600 μ L of ice-cold HBSS buffer. To measure the

Implications

This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.

Wiki pages this source may touch

• Fish — marine, non-predatory (sardines, anchovies, salmon, cod)
• Mercury
• Mercury
• Cadmium
• Arsenic

Verification notes

• Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing the linked reference record pages before creation.
• Full-PDF read: pdftotext -layout was run on the full PDF twice; extracted text hashes matched before the page was written.
• Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
• Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
• HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.

Update history

No substantive edit history is available in this build. The full commit record is available in git.