Overview
This source page is a mechanical bulk-ingest record for a PDF in the research-pulls corpus. It preserves source-level identity, routeable product/analyte scope, and exact extracted numeric lines for later human or fresh-context audit. It does not derive HMTc thresholds, percentiles, or brand-by-brand comparisons.
Key numbers
The worker extracted the full PDF text with layout preservation twice and compared extraction hashes before commit. The following lines are copied from numeric/table-bearing regions of the PDF and retain the source units and wording where legible:
- trations of these chemicals or their metabolites in the subjects’ urine over 48 h following exposure. Furthermore, we used long-range PCR to measure
- carriers excreted 18% and 110% more metabolite (estimated by AUCð0–40hÞ ) for pyrimethanil than wt carriers with low and high CNV, respectively.
- for chemical-related systemic effects, such as asthma (Paggiaro mately 2%–10% of Europeans carry at least one FLG null allele
- sequences are described in Table S1. The primers and probes were
- duced by Milli-Q® Integral 5 system, Millipore. FLG null allele carriers (n = 28) and an equal number of controls
- zone 6% of cosmetics according to http://ec.europa.eu/health/ph_ Exposure Experiment, including Sampling
- their informed consent (n = 445). Volunteers received a saliva
- tion. Saliva samples (n = 432) and questionnaires were sent by 8 extra wt carriers were included.
- Table 1. Characteristics, self-reported symptoms and diseases, and FLG null eyes from for example pollen or animals?”), and eczema (“Have
- Characteristic FLG null (n = 23) FLG wt (n = 31) p-Valuea you have eczema when you were a child?”).
- BMI (mean ± SD) 24:4 ± 5:8 24:1 ± 4:6 0.62 removed from the participants’ arms. The exposed areas were
- Sex washed three times with cotton swabs wetted with 70% ethanol.
- (1% w/v) with GelRed (Biotum) were loaded with PCR product
- The calibration standards ranged from 2 to 1,800 ng=mL for OH- (LOD) was 0:1 ng=mL for OH-pyrimethanil, 0:2 ng=mL for oxy-
- several chemical blanks, and quality control samples were The precisions are shown in Table S3, and the coefficients of var-
- included in each 96-well plate. Then, 25 lL of internal standard for iation were between 4% and 13%. The laboratory participates in
- for 10 min. For pyrimethanil, the median concentration in pre-exposure
- urine samples was <LOD ng=mL (57% < LOD) for FLG null
- LC-MS/MS Analysis carriers, and 0:4 ng=mL (35% < LOD) for FLG wt carriers; for
- 1-hydroxy-pyrene, the median was <LOD ng=mL (96% < LOD)
- The urine samples were analyzed using liquid chromatography for FLG null carriers, and <LOD ng=mL (90% < LOD) for FLG
- systems (UFLCRX, Shimadzu Corporation) coupled to triple wt carriers; and for oxybenzone, the median was 1:88 ng=mL
- quadrupole linear ion trap mass spectrometers (Sciex). Analysis (0% < LOD) for FLG null carriers, and 4:45 ng=mL (6% < LOD)
- shown in Table S2. All data acquisition and data processing were The laboratory is accredited for creatinine analysis and is using an
- 0.1% formic acid in Milli-Q® water and b) 0.1% formic acid in Toxicokinetic Analyses
- with 50% mobile phase B, followed by a linear gradient to 95% at 2010; Microsoft). The end time for urine collection differed
- 2.2 min and equilibrated at 50% B for 2 min. among the participants, so the AUC was calculated for 0–40 h.
- 60°C. The mobile phase gradient started with 75% mobile phase exposure were also omitted from the analysis (one wt carrier for
- brated at 75% B for 2 min. Dermal absorption and disposition were assessed by popula-
- tained at 60°C. The mobile phase gradient started with 20% mo- by an elimination rate constant (ke ), and exchange between the
- bile phase B, followed by a linear gradient to 95% at 2.0 min and central and peripheral compartments was described by two rate
- equilibrated at 20% B for 3 min. constants (k12 and k21 ). All rate constants are first order.
- the AUC analysis were also excluded from the toxicokinetic anal- The frequency of FLG null alleles was 6.5% in the entire
- CNV genotype were excluded from analysis (three wt carriers), (2.1%) for R501X, 3 (0.7%) for R2447X, 3 (0.7%) for S3247, and
- observational model was used as proposed by the software after tion of the applied doses (Table S4). A similar trend was found in
- (FLG null/wt, and FLG null/wt CNV20–22/wt CNV23–24) were nificant (Table S5). When grouping wt carriers according to
- 0 (Figures S1–S3) and the observed vs. predicted plots were suffi- CNV20–22 having 15% smaller AUCð0–40hÞ than FLG null and
- ciently evenly distributed around the line of unity (Figures S4–S6), wt CNV 23–24 having 44% smaller AUCð0–40hÞ than wt CNV20–
- altogether suggesting an adequate structural model. 22 to (Table 2). The same trend in AUCð0–40hÞ between FLG ge-
- Statistical Analysis but it was not statistically significant (p = 0:095) (Table S6).
- The wt carriers were further divided into two subgroups based on CNV23–24 carriers showed the lowest AUCð0–40hÞ (Table 2;
- their total CNV because we hypothesized that CNV affects skin Table S6).
Methods (brief)
- (IMM), Box 210, 17177 Stockholm, Sweden. Email: karin.broberg@ki.se carbons (PAHs) in the urine of a sample of chimney sweeps
- DNA was extracted from saliva samples from the 432 volunteers
- mass spectrometry (LC-MS/MS) and compared the levels of the
- Cosmetics Regulation); the pyrimethanil metabolite OH- reaction separately at a final concentration of 500 nM. Samples
- to, the type of samples that were going to be collected, and any risks
- samples and personal data are stored and that they could withdraw
- sample collection kit (Oragene DNA OG-500 kit, DNA Genotek)
- tion. Saliva samples (n = 432) and questionnaires were sent by 8 extra wt carriers were included.
- Previous smoker 8 (34.8) 9 (29.0) 0.57 16 500-mL bottles (VWR) to collect urine. Blood samples were
- Dry skin 15 (65.2) 17 (54.8) 0.31 were stored at −20 C. Study participants collected full urine
- Nickel allergy 1 (4.3) 6 (19.4) 0.21 approximately 48 h post exposure. Urine samples were kept in a
- 2282del4 10 (43.5) 0 NA sample were taken and stored at −20 C.
- chemicals, participants were given instructions not to consume I. Pre-exposure blood samples were extracted using the Omega
- experiment. The following samples were taken 10–30 min prior
- dissolving in methanol. A blank urine sample, obtained from a
- water. For quality control samples, the blank urine sample was
- 10 ng=mL for 1-hydroxy-pyrene. The quality control samples were for skin penetration (Tlag , h), first order skin absorption constant (Ka , h-1),
- Sample Preparation for LC-MS/MS rate constant (k, h−1 ).
Implications
This page makes the source discoverable for category-level evidence routing. Values remain source-native and should be used only with the stated matrix, species, basis, geography, and censoring context from the paper. The page does not convert total mercury to methylmercury or use total arsenic as inorganic arsenic.
Wiki pages this source may touch
- Fish — marine, non-predatory (sardines, anchovies, salmon, cod)
- Baby Sunscreen, Mineral (ZnO + TiO2)
- Nickel
- Aluminum
Verification notes
- Identity check: DOI, raw handle, candidate cite-key, and SHA-256 were compared against existing
wiki/sources/pages before creation. - Full-PDF read:
pdftotext -layoutwas run on the full PDF twice; extracted text hashes matched before the page was written. - Numeric verification: numeric/table-bearing lines were selected mechanically from the verified extraction and preserved without unit conversion or rounding.
- Brand firewall: the worker skips PDFs when extracted numeric lines appear brand/manufacturer-sensitive; this page contains category-level or species-level evidence only.
- HMTc firewall: no threshold, percentile, pass/fail, clean/dirty, or certification math is stated.
Update history
The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.