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Heavy Metal Index

Effect of Feed Additive on the Mineral Composition of Quail Blood

Overview

Kletikova and colleagues measured blood-serum mineral concentrations in control and feed-additive-treated Japanese laying quails. This is in-scope a4 animal biomonitoring and feed-additive exposure context, not pet-supplement or product occurrence.

Key numbers

The experimental group received a feed additive containing magnesium, B vitamins, and L-carnitine at 0.25 ml/liter of water for 120 days. At 120 days, blood serum was collected from 10 control quails and 10 experimental quails.

Table 1 reports macronutrients in blood serum, mg/l:

ParameterControl groupExperimental group
Calcium87.21 +/- 4.4683.31 +/- 0.18**
Potassium169.59 +/- 7.13165.73 +/- 2.24
Magnesium23.76 +/- 1.3425.15 +/- 0.11*
Sodium3200.03 +/- 114.323292.18 +/- 17.68*
Phosphorus78.98 +/- 4.1564.49 +/- 1.83**

Table 2 reports essential trace elements in blood serum, mg/l:

ParameterControl groupExperimental group
Iron1536.00 +/- 237.431345.60 +/- 21.32**
Copper762.40 +/- 38.56710.80 +/- 13.28**
Zinc707.18 +/- 46.84658.24 +/- 9.68**
Selenium128.80 +/- 13.37125.20 +/- 1.16
Molybdenum29.67 +/- 3.2324.09 +/- 1.27**
Chromium0.69 +/- 0.130.70 +/- 0.05
Manganese0.53 +/- 0.080.68 +/- 0.02*
Cobalt0.24 +/- 0.060.24 +/- 0.01

Table 3 reports roughly essential trace elements in blood serum, mg/l:

ParameterControl groupExperimental group
Silicon315.70 +/- 6.24301.70 +/- 4.38
Boron34.02 +/- 1.2334.59 +/- 1.46
Arsenic4.07 +/- 0.083.50 +/- 0.02**
Lithium3.30 +/- 0.334.60 +/- 0.28*
Nickel3.85 +/- 0.624.17 +/- 0.22*

Table 4 reports roughly toxic trace elements in blood serum, mg/l:

ParameterControl groupExperimental group
Antimony0.28 +/- 0.070.32 +/- 0.02*
Aluminum4.53 +/- 0.544.70 +/- 0.11
Titanium1.864 +/- 0.1342.236 +/- 0.026*
Lead0.31 +/- 0.080.36 +/- 0.02*
Mercury0.30 +/- 0.060.34 +/- 0.04*
Cadmium0.16 +/- 0.020.14 +/- 0.01

The abstract summarizes the feed-additive effect as decreases in phosphorus (18.30%), iron (12.29%), copper (6.76%), zinc (6.92%), molybdenum (18.80%), arsenic (14.00%), and cadmium (12.50%), and increases in magnesium (5.85%), manganese (28.31%), nickel (39.40%), lithium (8.32%), titanium (11.96%), lead (16.13%), mercury (13.34%), and antimony (14.29%) relative to the control group.

Methods (brief)

Two groups of one-day-old Japanese quails were raised during 2020-2021. The control group received pure boiled water, while the experimental group received the feed additive in water at 0.25 ml/l until 120 days. Blood was collected from the axillary vein before feeding. Trace elements were measured by mass spectrometry in a licensed laboratory; toxic elements and arsenic were analyzed under cited Russian GOST methods. Data were processed in Excel.

Update history

The five most recent substantive edits to this page, classified major (evidence or structure moved), correction (a published value or statement was wrong and has been fixed), or minor (narrative rewritten without changing the underlying evidence). Each description is derived from what the edit did to this page; the linked commit is the authoritative record, routine regeneration passes are excluded, and the full version history lives in git. When DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.

CommitDateChangeDescription
b01ec52c32026-08-04major1 section added
3171d069e2026-08-02major1 section added
90a2f9a382026-07-30major1 section added and 1 removed; narrative text revised
eaa8eeeed2026-07-27major1 section added
50de6ab212026-07-27minorNarrative text revised