Kletikova et al. 2022 - Quail blood minerals after feed additive
Kletikova and colleagues measured blood-serum mineral concentrations in control and feed-additive-treated Japanese laying quails. This is in-scope a4 animal biomonitoring and feed-additive exposure context, not pet-supplement or product occurrence.
Key numbers
The experimental group received a feed additive containing magnesium, B vitamins, and L-carnitine at 0.25 ml/liter of water for 120 days. At 120 days, blood serum was collected from 10 control quails and 10 experimental quails.
Table 1 reports macronutrients in blood serum, mg/l:
| Parameter | Control group | Experimental group |
|---|---|---|
| Calcium | 87.21 +/- 4.46 | 83.31 +/- 0.18** |
| Potassium | 169.59 +/- 7.13 | 165.73 +/- 2.24 |
| Magnesium | 23.76 +/- 1.34 | 25.15 +/- 0.11* |
| Sodium | 3200.03 +/- 114.32 | 3292.18 +/- 17.68* |
| Phosphorus | 78.98 +/- 4.15 | 64.49 +/- 1.83** |
Table 2 reports essential trace elements in blood serum, mg/l:
| Parameter | Control group | Experimental group |
|---|---|---|
| Iron | 1536.00 +/- 237.43 | 1345.60 +/- 21.32** |
| Copper | 762.40 +/- 38.56 | 710.80 +/- 13.28** |
| Zinc | 707.18 +/- 46.84 | 658.24 +/- 9.68** |
| Selenium | 128.80 +/- 13.37 | 125.20 +/- 1.16 |
| Molybdenum | 29.67 +/- 3.23 | 24.09 +/- 1.27** |
| Chromium | 0.69 +/- 0.13 | 0.70 +/- 0.05 |
| Manganese | 0.53 +/- 0.08 | 0.68 +/- 0.02* |
| Cobalt | 0.24 +/- 0.06 | 0.24 +/- 0.01 |
Table 3 reports roughly essential trace elements in blood serum, mg/l:
| Parameter | Control group | Experimental group |
|---|---|---|
| Silicon | 315.70 +/- 6.24 | 301.70 +/- 4.38 |
| Boron | 34.02 +/- 1.23 | 34.59 +/- 1.46 |
| Arsenic | 4.07 +/- 0.08 | 3.50 +/- 0.02** |
| Lithium | 3.30 +/- 0.33 | 4.60 +/- 0.28* |
| Nickel | 3.85 +/- 0.62 | 4.17 +/- 0.22* |
Table 4 reports roughly toxic trace elements in blood serum, mg/l:
| Parameter | Control group | Experimental group |
|---|---|---|
| Antimony | 0.28 +/- 0.07 | 0.32 +/- 0.02* |
| Aluminum | 4.53 +/- 0.54 | 4.70 +/- 0.11 |
| Titanium | 1.864 +/- 0.134 | 2.236 +/- 0.026* |
| Lead | 0.31 +/- 0.08 | 0.36 +/- 0.02* |
| Mercury | 0.30 +/- 0.06 | 0.34 +/- 0.04* |
| Cadmium | 0.16 +/- 0.02 | 0.14 +/- 0.01 |
The abstract summarizes the feed-additive effect as decreases in phosphorus (18.30%), iron (12.29%), copper (6.76%), zinc (6.92%), molybdenum (18.80%), arsenic (14.00%), and cadmium (12.50%), and increases in magnesium (5.85%), manganese (28.31%), nickel (39.40%), lithium (8.32%), titanium (11.96%), lead (16.13%), mercury (13.34%), and antimony (14.29%) relative to the control group.
Methods (brief)
Two groups of one-day-old Japanese quails were raised during 2020-2021. The control group received pure boiled water, while the experimental group received the feed additive in water at 0.25 ml/l until 120 days. Blood was collected from the axillary vein before feeding. Trace elements were measured by mass spectrometry in a licensed laboratory; toxic elements and arsenic were analyzed under cited Russian GOST methods. Data were processed in Excel.
Implications
Certification: Do not use these blood-serum values as pet-supplement occurrence, poultry meat occurrence, egg occurrence, or feed-product contamination. They are animal biomonitoring values after an experimental feed-additive exposure.
Courses: Useful example of why a feed additive can shift both essential and toxic element biomarkers in animals even when the additive is not framed as a contaminant study.
App: Context only. The source can support feed-additive exposure and animal biomarker narrative; it does not support finished product scoring.
Wiki pages this source may touch
Verification notes
Recovered from skip:not-food-occurrence under the 2026-06-10 inclusion-by-default rule. The old skip treated the paper as out of scope because it measured quail blood serum after feed additive exposure rather than product occurrence. On reading, it is in-scope a4 exposure/biomonitoring context with measured serum values.
Numbers were checked against the abstract, methods, Tables 1-4, and conclusion in the extracted PDF. Arsenic is recorded as tAs, mercury as tHg, and chromium as total Cr because the paper does not report speciation. Products and ingredients are intentionally empty.
Page history
The five most recent substantive edits to this page. The full version history lives in git; when DOI minting comes online (see schema docs), each entry below will also link to a version-pinned DataCite DOI.
| Commit | Date | Description |
|---|---|---|
| fa7f6a0 | 2026-06-11 | recover-ingest 2026-06-11: kletikova2022-quail-feed-blood-minerals (lane a4, was skip:not-food-occurrence) |